20.500.12556/DiRROS-20262 Guidance document on multiplex real-time PCR methods European Network of GMO Laboratories This document provides guidance for the development of new qualitative and quantitative multiplex real-time methods (mpPCR) and their implementation in a routine testing laboratory for food, feed and seeds. When implementing a mpPCR method, the laboratory should perform either an (in-house) validation or a verification of the method before its use. The various method performance parameters of the specific requirements for mpPCR are described and examples are given. Several different aspects have been considered, including the selection of the fluorophores, the master mix and the real-time PCR instrument used. The technical aspects are examined and recommendations are made on the choice of the detection and quantification strategy. Finally, a separate chapter is dedicated to troubleshooting (e.g. underperforming method, no or poor signal strength, cross-talk). In addition, a collection of existing mpPCR methods with emphasis on GMO detection is presented. true false true Publications Office of the European Union Angleški jezik Ni določen Temeljna raziskava 2024-09-02 12:06:44 2024-09-02 12:06:44 2024-09-04 03:35:48 0000-00-00 00:00:00 2021 0 Luxembourg 2021 Luxembourg Soavtorji: Barbante A, Eriksson R, Gatto F, Georgieva T, Huber I, Hulin J, Köppel R, Marchesi U, Marmin L, Mazzara M, Narendja F, Owen H, Perri E, Scholtens I, Sovová T, Sowa S, Stebih D, Weidner C, Zdeňková K.; Nasl. z nasl. zaslona; Opis vira z dne 19. 1. 2022; 1 spletni vir (1 datoteka PDF ([62] str.)) 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-01-01 577 978-92-76-37820-4 10.2760/243914 94024963 https://data.europa.eu/doi/10.2760/243914 1 52ef19bb-6902-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28403 https://op.europa.eu/publication/manifestation_identifier/PUB_KJNA30708ENN 1 55dbd06a-6902-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28404 RAZ_Grohmann_Lutz_2021.pdf RAZ_Grohmann_Lutz_2021.pdf 1 CD406107C42F36D239E62D0C1552182D 920484e24bd4b6465dfb91a5f2b3d5fc38094973fc3cc0a9d2a0787acbff23d1 7caf3dbd-6902-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28405 http://dx.doi.org/10.2760/243914 0 8729a696-6902-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28406 Nacionalni inštitut za biologijo 0 0 0