20.500.12556/DiRROS-20241 Cyanobacterial cyclic peptides can disrupt cytoskeleton organization in human astrocytes a contribution to the understanding of the systemic toxicity of cyanotoxins Cyanobacterial cyclic peptides can disrupt cytoskeleton organization in human astrocytes – a contribution to the understanding of the systemic toxicity of cyanotoxins The systemic toxicity of cyclic peptides produced by cyanobacteria (CCPs) is not yet completely understood. Apart from the most known damages to the liver and kidneys, symptoms of their neurotoxicity have also been reported. Hepatotoxic CCPs, like microcystins, as well as non-hepatotoxic anabaenopeptins and planktopeptins, all exhibit cytotoxic and cytostatic effects on mammalian cells. However, responses of different cell types to CCPs depend on their specific modes of interaction with cell membranes. This study demonstrates that non-hepatotoxic planktopeptin BL1125 and anabaenopeptins B and F, at concentrations up to 10 µM, affect normal and tumor human astrocytes (NHA and U87-GM) in vitro by their almost immediate insertion into the lipid monolayer. Like microcystin-LR (up to 1 µM), they inhibit Ser/Thr phosphatases and reorganize cytoskeletal elements, with modest effects on their gene expression. Based on the observed effects on intermediate filaments and intermediate filament linkage elements, their direct or indirect influence on tubulin cytoskeletons via post-translational modifications, we conclude that the basic mechanism of CCP toxicities is the induction of inter- and intracellular communication failure. The assessed inhibitory activity on Ser/Thr phosphatases is also crucial since the signal transduction cascades are modulated by phosphorylation/dephosphorylation processes. astrocytes cyclic cyanobacterial peptides cytoskeletal organization Ser/Thr phosphatases systemic toxicity cyanobacteria astrociti ciklični cianobakterijski peptidi citoskeletna organizacija Ser/Thr fosfataze sistemska toksičnost cianobakterije true false true Angleški jezik Slovenski jezik Neznano 2024-08-28 12:07:11 2024-08-28 12:07:11 2024-08-29 03:35:38 0000-00-00 00:00:00 2024 0 0 Soavtorji: Robert Frangež, Monika C. Žužek, Ion Gutiérrez-Aguirre, Tamara T. Lah and Bojan Sedmak; Nasl. z nasl. zaslona; Opis vira z dne 28. 8. 2024; str. 1-18 [art no.] ǂ374 iss. 9 Sept. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-09-01 576 2072-6651 10.3390/toxins16090374 205590019 https://doi.org/10.3390/toxins16090374 1 88e16578-6514-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28358 RAZ_Bubik_Anja_2024.pdf RAZ_Bubik_Anja_2024.pdf 1 E7C76924AF8B510314F095D2393A909B 02723a4cba0d267d5fded473693e2b8f3c812f5e13b14cf041ceddeff8ce2f87 ce839dc9-6514-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28359 Nacionalni inštitut za biologijo 0 0 0