20.500.12556/DiRROS-20183 Synthesis, purification, and cell-toxicity of a choline betainate In this work, choline chloride and betaine hydrochloride were condensed into a - to our knowledge - unreported choline betainate (N,N,N-trimethyl-2-oxo-2-(2-(trimethylammonio)ethoxy)ethanaminium chloride) using 1,1′-carbonyldiimidazole (CDI) activation of betaine hydrochloride in dimethylsulfoxide. The product and reaction intermediates were isolated, purified by preparative HPLC and analyzed in detail by infrared and nuclear magnetic resonance spectroscopy. The final product has a high cytotoxicity for L929 mouse fibroblasts, and low antibacterial activity against P. Aeruginosa and S. Aureus at concentrations of up to 20 mg/ml. It could potentially further be investigated for similar uses as suxamethonium chloride, a muscle relaxant drug. choline chloride betaine hydrochloride carbonyldiimidazole HPLC antimicrobial cytotoxicity molekularna biologija holin klorid betain hidroklorid karbonildiimidazol protimikrobnost cititoksičnost true false true Angleški jezik Slovenski jezik Neznano 2024-08-07 12:49:06 2024-08-07 12:49:07 2024-08-08 04:07:53 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Soavtorji: Gregor Hostnik , Tobias Steindorfer, Alja Štern, Perica Bošković, Matej Bračič, Bojana Žegura, Rupert Kargl; In Press, Journal pre-proof; Opis vira z dne 16. 5. 2024; [16] str. [article no.] 138581 Available online 8 May 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-09-15 577:576 1872-8014 10.1016/j.molstruc.2024.138581 195589379 RAZ_Jurko_Lucija_2024.pdf RAZ_Jurko_Lucija_2024.pdf 1 F137BF01D4EA39F4FFE15311D3926E37 8ecbdb7a330aeb0496a4626774d47c48c7e59f806f969a250f0620fd259f1752 061de55d-549a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28230 https://doi.org/10.1016/j.molstruc.2024.138581 0 0e202c44-549a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28231 Nacionalni inštitut za biologijo 0 0 0