20.500.12556/DiRROS-20180 Hepatic alterations in a BTBR T + Itpr3tf/J mouse model of autism and improvement using melatonin via mitigation oxidative stress, inflammation and ferroptosis Autism spectrum disorder (ASD) is a complicated neurodevelopmental disorder, and its etiology is not well understood. It is known that genetic and nongenetic factors determine alterations in several organs, such as the liver, in individuals with this disorder. The aims of the present study were to analyze morphological and biological alterations in the liver of an autistic mouse model, BTBR T + Itpr3tf/J (BTBR) mice, and to identify therapeutic strategies for alleviating hepatic impairments using melatonin administration. We studied hepatic cytoarchitecture, oxidative stress, inflammation and ferroptosis in BTBR mice and used C57BL6/J mice as healthy control subjects. The mice were divided into four groups and then treated and not treated with melatonin, respectively. BTBR mice showed (a) a retarded development of livers and (b) iron accumulation and elevated oxidative stress and inflammation. We demonstrated that the expression of ferroptosis markers, the transcription factor nuclear factor erythroid-related factor 2 (NFR2), was upregulated, and the Kelch-like ECH-associated protein 1 (KEAP1) was downregulated in BTBR mice. Then, we evaluated the effects of melatonin on the hepatic alterations of BTBR mice; melatonin has a positive effect on liver cytoarchitecture and metabolic functions. autism spectrum disorder liver oxidative stress inflammation ferroptosis true false true Angleški jezik Ni določen Neznano 2024-08-07 12:33:12 2024-08-07 12:33:13 2024-08-08 04:07:53 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Soavtorji: Marzia Gianò, Daniela Pinto, Fabio Rinaldi, Cornelis J. F. van Noorden and Gaia Favero; Opis vira z dne 31. 3. 2024; str. 1-20 no. 2, [article no.] 1086 Vol. 25 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-01-16 577.2 1422-0067 10.3390/ijms25021086 190985987 https://www.mdpi.com/1422-0067/25/2/1086 1 b1f371ad-5497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28222 RAZ_Rezzani_Rita_2024.pdf RAZ_Rezzani_Rita_2024.pdf 1 335DEDB060BD841A9D8D6311D16F8742 769104a9b15e4daf6cc660d30ff531fdb3d3a15e3a87ec228909c929f61b7840 c8f6327e-5497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28223 https://doi.org/10.3390/ijms25021086 0 d048623f-5497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28224 Nacionalni inštitut za biologijo 0 0 0