20.500.12556/DiRROS-20179 Retrospective survey of Dickeya fangzhongdai using a novel validated real-time PCR assay Dickeya fangzhongdai, an aggressive plant pathogen, causes symptoms on a variety of crops and ornamental plants including bleeding canker of Asian pear trees. Historical findings stress the need for a specific detection tool for D. fangzhongdai to prevent overlooking the pathogen or assigning it to general Dickeya spp. Therefore, a qualitative real-time PCR for specific detection of D. fangzhongdai has been developed and validated. The developed assay shows selectivity of 100%, diagnostic sensitivity of 76% and limit of detection with 95% confidence interval in plant matrices ranging from 311 to 2,275 cells/mL of plant extracts. The assay was successfully used in a retrospective survey of selected host plants of relevance to Europe and environmental niches relevant to D. fangzhongdai. Samples of potato tubers and plants, plants from the Malinae subtribe (apple, pear, quince, and Asian pear tree) and fresh surface water from Slovenia were analyzed. D. fangzhongdai was not detected in any plant samples, however, 12% of surface water samples were found to be positive. molecular testing diagnostics plant pathogen real-time PCR Dickeya survey water molekularno testiranje diagnostika rastlinski patogen PCR v realnem času raziskava voda true false true Angleški jezik Slovenski jezik Neznano 2024-08-07 12:26:44 2024-08-07 12:26:44 2024-08-08 04:07:53 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 27. 3. 2024; str. 1-15 [art. no.] ǂ1249955 Vol. 14 Feb. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-02-13 577 1664-302X 10.3389/fmicb.2023.1249955 190578179 https://doi.org/10.3389/fmicb.2023.1249955 1 ca714933-5496-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28220 RAZ_Alic_Spela_2024.pdf RAZ_Alic_Spela_2024.pdf 1 8E7A8603460A2E1675A97B90F44A8790 eaf2031840d1f9b4f35a3b8cd2fa8b3458cf68a2374e6dfac01ba681951aca77 f815c33f-5496-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28221 Nacionalni inštitut za biologijo 0 0 0