20.500.12556/DiRROS-20139 Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products The emergence of high-throughput, massive or next-generation sequencing technologies has created a completely new foundation for molecular analyses. Various selective enrichment processes are commonly applied to facilitate detection of predefined (known) targets. Such approaches, however, inevitably introduce a bias and are prone to miss unknown targets. Here we review the application of high-throughput sequencing technologies and the preparation of fit-for-purpose whole genome shotgun sequencing libraries for the detection and characterization of genetically modified and derived products. The potential impact of these new sequencing technologies for the characterization, breeding selection, risk assessment, and traceability of genetically modified organisms and genetically modified products is yet to be fully acknowledged. The published literature is reviewed, and the prospects for future developments and use of the new sequencing technologies for these purposes are discussed. cisgene intragene traceability traceability transcriptome sequencing transgene unknown GMO true false true Angleški jezik Ni določen Neznano 2024-08-06 10:13:04 2024-08-06 10:13:05 2024-08-08 03:48:00 0000-00-00 00:00:00 2016 0 0 str. 4595-4614 iss. 17 Vol. 408 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2016-04-21 577.2 1618-2642 10.1007/s00216-016-9549-1 3852879 RAZ_Holst-Jensen_Arne_2016.pdf RAZ_Holst-Jensen_Arne_2016.pdf 1 1CF530CB5B5A861F9CD3D2D24AD26F29 8c2e91b6cd0f896a2393753e33c948cf649980b0a00de02be767c9a767452c5d 372c51d1-53bb-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28118 https://doi.org/10.1007/s00216-016-9549-1 0 44763f61-53bb-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28119 Nacionalni inštitut za biologijo 0 0 0