20.500.12556/DiRROS-20106 Stem cells of aquatic invertebrates as an advanced tool for assessing ecotoxicological impacts Environmental stressors are assessed through methods that quantify their impacts on a wide range of metrics including species density, growth rates, reproduction, behaviour and physiology, as on host-pathogen interactions and immunocompetence. Environmental stress may induce additional sublethal effects, like mutations and epigenetic signatures affecting offspring via germline mediated transgenerational inheritance, shaping phenotypic plasticity, increasing disease susceptibility, tissue pathologies, changes in social behaviour and biological invasions. The growing diversity of pollutants released into aquatic environments requires the development of a reliable, standardised and 3R (replacement, reduction and refinement of animals in research) compliant in vitro toolbox. The tools have to be in line with REACH regulation 1907/2006/EC, aiming to improve strategies for potential ecotoxicological risks assessment and monitoring of chemicals threatening human health and aquatic environments. Aquatic invertebrates' adult stem cells (ASCs) are numerous and can be pluripotent, as illustrated by high regeneration ability documented in many of these taxa. This is of further importance as in many aquatic invertebrate taxa, ASCs are able to differentiate into germ cells. Here we propose that ASCs from key aquatic invertebrates may be harnessed for applicable and standardised new tests in ecotoxicology. As part of this approach, a battery of modern techniques and endpoints are proposed to be tested for their ability to correctly identify environmental stresses posed by emerging contaminants in aquatic environments. Consequently, we briefly describe the current status of the available toxicity testing and biota-based monitoring strategies in aquatic environmental ecotoxicology and highlight some of the associated open issues such as replicability, consistency and reliability in the outcomes, for understanding and assessing the impacts of various chemicals on organisms and on the entire aquatic environment. Following this, we describe the benefits of aquatic invertebrate ASC-based tools for better addressing ecotoxicological questions, along with the current obstacles and possible overhaul approaches. odrasle zarodne celice sladkovodni in morski nevretenčarji in vitro celične kulture ekotoksikologija true false true Angleški jezik Slovenski jezik Neznano 2024-08-05 16:00:15 2024-08-05 16:00:15 2024-08-07 03:58:16 0000-00-00 00:00:00 2021 0 0 Soavtorji: Armengaud Jean, Ballarin Loriano, Barnay-Verdier Stéphanie, Cima Francesca, Coelho Ana Varela Domart-Coulon Isabelle, Drobne Damjana, Genevière AnneMarie, Jemec Kokalj Anita, Kotlarska Ewa, Lyons Daniel Mark, Mass Tali, Paz Guy, Pazdro Ksenia, Perić Lorena, Ramšak Andreja, Rakers Sebastian, Rinkevich Baruch, Spagnuolo Antonietta, Sugni Michela, Cambier Sébastien; Št. e-članka: 144565; str. [1-23] Vol. 771 1 Jun. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-06-01 574 0048-9697 10.1016/j.scitotenv.2020.144565 48752387 https://www.sciencedirect.com/science/article/pii/S0048969720380967?via%3Dihub#! 1 48c5e580-5322-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28030 RAZ_Rosner_Amalia_2021.pdf RAZ_Rosner_Amalia_2021.pdf 1 D4B05BADDA300FAC64E20F779F977CDD 608d86dcdcc009b4d14ad6ef229c6461c9e36ca66d564735839e5df8725ffceb 82772a01-5322-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28031 https://doi.org/10.1016/j.scitotenv.2020.144565 0 89d6822f-5322-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28032 Nacionalni inštitut za biologijo 0 0 0