20.500.12556/DiRROS-20104 Guidelines for the reliable use of high throughput sequencing technologies to detect plant pathogens and pests High-throughput sequencing (HTS) technologies have the potential to become one of the most significant advances in molecular diagnostics. Their use by researchers to detect and characterize plant pathogens and pests has been growing steadily for more than a decade and they are now envisioned as a routine diagnostic test to be deployed by plant pest diagnostics laboratories. Nevertheless, HTS technologies and downstream bioinformatics analysis of the generated datasets represent a complex process including many steps whose reliability must be ensured. The aim of the present guidelines is to provide recommendations for researchers and diagnosticians aiming to reliably use HTS technologies to detect plant pathogens and pests. These guidelines are generic and do not depend on the sequencing technology or platform. They cover all the adoption processes of HTS technologies from test selection to test validation as well as their routine implementation. A special emphasis is given to key elements to be considered: undertaking a risk analysis, designing sample panels for validation, using proper controls, evaluating performance criteria, confirming and interpreting results. These guidelines cover any HTS test used for the detection and identification of any plant pest (viroid, virus, bacteria, phytoplasma, fungi and fungus-like protists, nematodes, arthropods, plants) from any type of matrix. Overall, their adoption by diagnosticians and researchers should greatly improve the reliability of pathogens and pest diagnostics and foster the use of HTS technologies in plant health. bolezni rastlin rastlinski virusi rastlinski patogeni rastlinski škodljivci sekvenciranje true false true Angleški jezik Slovenski jezik Neznano 2024-08-05 15:38:01 2024-08-05 15:38:01 2024-08-07 03:53:27 0000-00-00 00:00:00 2022 0 0 Nasl. z nasl. zaslona; Opis vira z dne 11. 11. 2022; Online 25. 10. 2022; Št. članka: e62; str. 1-34 Vol. 2 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-10-25 632 2804-3871 10.24072/pcjournal.181 128955139 https://peercommunityjournal.org/articles/10.24072/pcjournal.181/ 1 2eb5f99b-531f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28025 RAZ_Massart_Sebastien_2022.pdf RAZ_Massart_Sebastien_2022.pdf 1 58C2A254270B6E6C36FEE6309F60C94B 8700367cd84d164e847fce3fe131cb7e5eb232ceae2b9a8ea3ce0092dd60cc46 6eec45f7-531f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28026 https://doi.org/10.24072/pcjournal.181 0 8399cda4-531f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28027 Nacionalni inštitut za biologijo 0 0 0