20.500.12556/DiRROS-20070 Molecular screening for cyanobacteria and their cyanotoxin potential in diverse habitats Cyanobacteria are adaptable and dominant organisms that exist in many harsh and extreme environments due to their great ecological tolerance. They produce various secondary metabolites, including cyanotoxins. While cyanobacteria are well studied in surface waters and some aerial habitats, numerous other habitats and niches remain underexplored. We collected 61 samples of: (i) biofilms from springs, (ii) aerial microbial mats from buildings and subaerial mats from caves, and (iii) water from borehole wells, caves, alkaline, saline, sulphidic, thermal, and iron springs, rivers, seas, and melted cave ice from five countries (Croatia, Georgia, Italy, Serbia, and Slovenia). We used (q)PCR to detect cyanobacteria (phycocyanin intergenic spacer—PC-IGS and cyanobacteria-specific 16S rRNA gene) and cyanotoxin genes (microcystins—mcyE, saxitoxins—sxtA, cylindrospermopsins—cyrJ), as well as amplicon sequencing and morphological observations for taxonomic identification. Cyanobacteria were detected in samples from caves, a saline spring, and an alkaline spring. While mcyE or sxtA genes were not observed in any sample, cyrJ results showed the presence of a potential cylindrospermopsin producer in a biofilm from a sulphidic spring in Slovenia. This study contributes to our understanding of cyanobacteria occurrence in diverse habitats, including rare and extreme ones, and provides relevant methodological considerations for future research in such environments. extreme environments cylindrospermopsin sulphidic springs caves qPCR PC-IGS ekstremna okolja cilindrospermopsini sulfidni izviri jame qPCR molekularna biologija true false true Angleški jezik Slovenski jezik Neznano 2024-08-05 12:55:37 2024-08-05 12:55:37 2024-09-09 17:37:29 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 5. 8. 2024; str. 1-20 iss. 8, [article no.] 333 Vol. 16 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-07-27 577 2072-6651 10.3390/toxins16080333 203590659 https://www.mdpi.com/2072-6651/16/8/333 1 7e4c21b5-5308-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27967 RAZ_Jablonska_Masa_2024.pdf RAZ_Jablonska_Masa_2024.pdf 1 CD0085D90E81DCEBA1D7C61802FC61F5 a5e1d08c2317b43e72f045076efe1a660db4bcfa36eb1186f7378bcc04f0795a e998a384-5308-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27970 Nacionalni inštitut za biologijo 0 0 0