20.500.12556/DiRROS-20050 Programmed cell death occurs asymmetrically during abscission in tomato Abscission occurs specifically in the abscission zone (AZ) tissue as a natural stage of plant development. Previously, we observed delay of tomato (Solanum lycopersicum) leaf abscission when the LX ribonuclease (LX) was inhibited. The known association between LX expression and programmed cell death (PCD) suggested involvement of PCD in abscission. In this study, hallmarks of PCD were identified in the tomato leaf and flower AZs during the late stage of abscission. These included loss of cell viability, altered nuclear morphology, DNA fragmentation, elevated levels of reactive oxygen species and enzymatic activities, and expression of PCD-associated genes. Overexpression of antiapoptotic proteins resulted in retarded abscission, indicating PCD requirement. PCD, LX, and nuclease gene expression were visualized primarily in the AZ distal tissue, demonstrating an asymmetry between the two AZ sides. Asymmetric expression was observed for genes associated with cell wall hydrolysis, leading to AZ, or associated with ethylene biosynthesis, which induces abscission. These results suggest that different abscission-related processes occur asymmetrically between the AZ proximal and distal sides. Taken together, our findings identify PCD as a key mechanism that occurs asymmetrically during normal progression of abscission and suggest an important role for LX in this PCD process. plant cell cell death abscission true false true Angleški jezik Ni določen Neznano 2024-08-05 10:06:21 2024-08-05 10:06:22 2024-08-06 04:00:45 0000-00-00 00:00:00 2011 0 0 str. 4146-4163 no. 11 Vol. 23 2011 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2011-11-01 577.2 1040-4651 10.1105/tpc.111.092494 2469199 RAZ_Bar-Dror_Tal_2011.pdf RAZ_Bar-Dror_Tal_2011.pdf 1 423050ED60F8C13334288E9E2C7BD5E9 94aff0bd5bfa67ec03b13f5a483bb6451d753d3abe01526e47dfa0173c231034 47ea9482-52f1-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27932 https://doi.org/10.1105/tpc.111.092494 0 51bb1ff0-52f1-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27933 Nacionalni inštitut za biologijo 0 0 0