20.500.12556/DiRROS-20049 Effect of poly-[alpha], [gamma], L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species. green synthesis morphology cytotoxicity true false true Angleški jezik Ni določen Neznano 2024-08-05 10:02:23 2024-08-05 10:02:24 2024-08-06 04:00:44 0000-00-00 00:00:00 2011 0 0 str. 2837-2847 Vol. 6 2011 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2011-11-15 577 1178-2013 10.2147/IJN.S24889 2459983 http://dx.doi.org/10.2147/IJN.S24889 1 4d03c6d8-52f0-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27930 RAZ_Stevanovic_Magdalena_2011.pdf RAZ_Stevanovic_Magdalena_2011.pdf 1 3E6433669FF18268A146B09B229227B1 eea9056d554a591e29a18362f2f23d3927460d5d0f4ca4c10a28678d5d4aca63 6e5e4bae-52f0-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27931 Nacionalni inštitut za biologijo 0 0 0