20.500.12556/DiRROS-20036 Gene expression profiling of recombinant protein producing E. coli at suboptimal growth temperature Recent studies have revealed that at lower cultivation temperatures (25 °C) much higher percentage of correctly folded recombinant hG-CSF protein can be extracted from inclusion bodies. Hence, the goal of our research was to investigate mechanisms determining characteristics of non-classical inclusion bodies production using gene expression profiling, focusing on proteases and chaperones gene expression. Statistical analysis of microarray data showed prominent changes in energy metabolism, in metabolism of amino acids and nucleotides, as well as in biosynthesis of cofactors and secondary metabolites if the culture was grown below its optimal temperature. Moreover, 24 differentially expressed up to now known genes classified among proteases, chaperones and other heat or stress related genes. Among chaperones UspE and among proteases YaeL and YeaZ might play an important role in accumulation of correctly folded recombinant proteins. Membrane localized protease yaeL gene was found to have higher activity at 25 °C and is thus potentially functionally related to the more efficient recombinant protein production at lower temperatures. The results of this study represent advance in the understanding of recombinant protein production in E. coli. Genes potentially influencing production of recombinant protein at lower growth temperature represent basis for further research towards improvement of E. coli production strains as well as fermentation process. recombinant protein production non-classical inclusion bodies expression microarrays YaeL protease GroEL chaperone true false true Angleški jezik Slovenski jezik Neznano 2024-08-05 07:56:15 2024-08-05 07:56:15 2024-08-06 04:00:36 0000-00-00 00:00:00 2012 0 0 str. 59-69 no. 1 Vol. 59 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2012-01-01 577.2 1318-0207 2543695 http://acta.chem-soc.si/59/59-1-59.pdf 1 c6687e3b-52de-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27899 RAZ_Mahnic_Mitja_2012.pdf RAZ_Mahnic_Mitja_2012.pdf 1 4CD37E1201E8896A29D0664B3E70BFE0 4e799e513b9a5c6b3709ef3bf2f94e59650793076a3273e6a2be9a166417fa84 2460ad6f-52df-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27900 https://www.dlib.si/details/URN:NBN:SI:doc-DNNR3CPE 0 2d20f789-52df-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27901 Nacionalni inštitut za biologijo 0 0 0