20.500.12556/DiRROS-20027 Insertion of a specific fungal 3'-phosphoadenosine-5'-phosphatase motif into a plant homologue improves halotoleranceand drought tolerance of plants Soil salinity and drought are among the most serious agricultural and environmental problems of today. Therefore, investigations of plant resistance to abiotic stress have received a lot of attention in recent years. In this study, we identified the complete coding sequence of a 3′-phosphoadenosine-5′-phosphatase protein, ApHal2, from the halotolerant yeast Aureobasidium pullulans. Expression of the ApHAL2 gene in a Saccharomyces cerevisiae hal2 mutant complemented the mutant auxotrophy for methionine, and rescued the growth of the hal2 mutant in media with high NaCl concentrations. A 21-amino-acids-long region of the ApHal2 enzyme was inserted into the Arabidopsis thaliana homologue of Hal2, the SAL1 phosphatase. The inserted sequence included the META motif, which has previously been implicated in increased sodium tolerance of the Hal2 homologue from a related fungal species. Transgenic Arabidopsis plants overexpressing this modified SAL1 (mSAL1) showed improved halotolerance and drought tolerance. In a medium with an elevated salt concentration, mSAL1-expressing plants were twice as likely to have roots in a higher length category in comparison with the wild-type Arabidopsis and with plants overexpressing the native SAL1, and had 5% to 10% larger leaf surface area under moderate and severe salt stress, respectively. Similarly, after moderate drought exposure, the mSAL1-expressing plants showed 14% increased dry weight after revitalisation, with no increase in dry weight of the wild-type plants. With severe drought, plants overexpressing native SAL1 had the worst rehydration success, consistent with the recently proposed role of SAL1 in severe drought. This was not observed for plants expressing mSAL1. Therefore, the presence of this fungal META motif sequence is beneficial under conditions of increased salinity and moderate drought, and shows no drawbacks for plant survival under severe drought. This demonstrates that adaptations of extremotolerant fungi should be considered as a valuable resource for improving stress-tolerance in plant breeding in the future. soil salinity and drought plant resistance abiotic stress slanost in suša tal odpornost rastlin abiotski stres true false true Angleški jezik Slovenski jezik Neznano 2024-08-02 11:43:46 2024-08-02 11:43:46 2024-08-03 03:45:59 0000-00-00 00:00:00 2013 0 0 Nasl. z nasl. zaslona; Opis vira z dne 11. 12. 2013; Članek v pdf obsega 12 str.; iss. 12 Vol. 8 Dec. 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2013-12-09 577 1932-6203 10.1371/journal.pone.0081872 31021785 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0081872 1 f8487919-50a2-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27880 RAZ_Buh_Gasparic_Meti_2013.pdf RAZ_Buh_Gasparic_Meti_2013.pdf 1 6F196ED69E4D9BB28BCBB19450C4C9BA e429a78ce2b1e3c745ff5c1e9cfa31586fa0a44b37e963beec7920e868a93870 7f34e174-50a3-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27881 https://doi.org/10.1371/journal.pone.0081872 0 887ad644-50a3-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27882 Nacionalni inštitut za biologijo 0 0 0