20.500.12556/DiRROS-20024 The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident that cultivation and molecular methods are complementary and in these cases highly relevant. We report a simple and efficient method for sampling and analyzing airborne bacteria with an impactor-type high-flow-rate portable air sampler, currently used for monitoring culturable bacteria or fungi. A method is reported for extraction of nucleic acids from impacted cells without prior cultivation and using agarose as a sampling matrix. The DNA extraction efficiency was determined in spiked samples and, samples taken from a wastewater treatment plant and an alpine area. The abundance, diversity and quantity of total bacteria were analysed by a quantitative polymerase chain reaction, and by construction and analysis of clone libraries. The method does not interfere with downstream PCR analysis and can cover the gap between traditional culture and molecular techniques of bioaerosol monitoring. true false true Angleški jezik Ni določen Neznano 2024-08-02 11:26:18 2024-08-02 11:26:19 2024-08-03 03:45:59 0000-00-00 00:00:00 2013 0 0 avtorski PDF; e82186; Str. 1-9; e82186 št. 12 Vol. 8 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2013-12-20 579.6 1932-6203 10.1371/journal.pone.0082186 3029583 RAZ_Hubad_Barbara_2013.pdf RAZ_Hubad_Barbara_2013.pdf 1 88B4E2499807F7F20BA1115A0E27350D 581620f7fac9037e96ec102805a75f06fa7230604f3b68ca30a3990fc814ddae 10bd91a3-50a1-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27874 https://doi.org/10.1371/journal.pone.0082186 0 1a9bac2e-50a1-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27875 Nacionalni inštitut za biologijo 0 0 0