20.500.12556/DiRROS-20017 CellTracker Green labelling vs. Rose Bengal staining: CTG wins by points in distinguishing living from dead anoxia-impacted copepods and nematods Hypoxia and anoxia have become a key threat to shallow coastal seas. Much is known about their impact on macrofauna, less on meiofauna. In an attempt to shed more light on the latter group, in particular from a process-oriented view, we experimentally induced short-term anoxia (1 week) in the northern Adriatic Sea (Mediterranean) and examined the two most abundant meiofauna taxa – harpacticoid copepods and nematodes. Both taxa also represent different ends of the tolerance spectrum, with copepods being the most sensitive and nematodes among the most tolerant. We compared two methods: CellTracker Green (CTG) – new labelling approach for meiofauna – with the traditional rose bengal (RB) staining method. CTG binds to active enzymes and therefore colours live organisms only. The two methods show considerable differences in the number of living and dead individuals of both meiofauna taxa. Generally, RB will stain dead but not yet decomposed copepods and nematodes equally as it does live ones. Specifically, RB significantly overestimated the number of living copepods in all sediment layers in anoxic samples, but not in any normoxic samples. In contrast, for nematodes, the methods did not show such a clear difference between anoxia and normoxia. RB overestimated the number of living nematodes in the top sediment layer of normoxic samples, which implies an overestimation of the overall live nematofauna. For monitoring and biodiversity studies, the RB method might be sufficient, but for more precise quantification of community degradation, especially after an oxygen depletion event, CTG labelling is a better tool. Moreover, it clearly highlights the surviving species within the copepod or nematode community. As already accepted for foraminiferal research, we demonstrate that the CTG labelling is also valid for other meiofauna groups. morje plankton kopepodi živalski plankton trofični status morski plankton CTG CellTracker Green true false true Angleški jezik Ni določen Jadransko morje; Neznano 2024-08-02 09:56:48 2024-08-02 09:56:48 2024-08-03 03:45:59 0000-00-00 00:00:00 2013 0 0 Izv. znanstv. članek; Predčasna objava DOI v BGD februarja 2013, BG jul. 2013; končna objava v BG dne 10. julija 2013; str. 4565-4575 no. 7 Vol. 10 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2013-07-09 574 1726-4170 10.5194/bg-10-4565-2013 2809423 RAZ_Grego_Mateja_2013.pdf RAZ_Grego_Mateja_2013.pdf 1 976BF6DC98EC1365D7F821B8FDCB53FC 0b84f260fdb006fd23253910292183c80a245f234972a111598806431ae08c9c a1782713-5094-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27851 https://doi.org/10.5194/bg-10-4565-2013 0 ae088387-5094-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27852 Nacionalni inštitut za biologijo 0 0 0