20.500.12556/DiRROS-20004 Mixtures of Chemical Pollutants at European Legislation Safety Concentrations: How Safe are They? The risk posed by complex chemical mixtures in the environment to wildlife and humans is increasingly debated, but has been rarely tested under environmentally relevant scenarios. To address this issue, two mixtures of 14 or 19 substances of concern (pesticides, pharmaceuticals, heavy metals, polyaromatic hydrocarbons, a surfactant, and a plasticizer), each present at its safety limit concentration imposed by the European legislation, were prepared and tested for their toxic effects. The effects of the mixtures were assessed in 35 bioassays, based on 11 organisms representing different trophic levels. A consortium of 16 laboratories was involved in performing the bioassays. The mixtures elicited quantifiable toxic effects on some of the test systems employed, including i) changes in marine microbial composition, ii) microalgae toxicity, iii) immobilization in the crustacean Daphnia magna, iv) fish embryo toxicity, v) impaired frog embryo development, and vi) increased expression on oxidative stress-linked reporter genes. Estrogenic activity close to regulatory safety limit concentrations was uncovered by receptor-binding assays. The results highlight the need of precautionary actions on the assessment of chemical mixtures even in cases where individual toxicants are present at seemingly harmless concentrations. morje analizna kemija biologija evtrofikacija hranilne snovi fitoplankton onesnaževanje Piranski zaliv true false true Angleški jezik Ni določen Jadransko morje; Neznano 2024-08-02 08:03:50 2024-08-02 08:03:50 2024-08-03 03:45:57 0000-00-00 00:00:00 2014 0 0 str. 218-233 issue 1 Vol. 141 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2014-09-12 543 1096-6080 10.1093/toxsci/kfu118 3160655 http://toxsci.oxfordjournals.org/content/early/2014/06/23/toxsci.kfu118.abstract?sid=d8381fd4-bf03-4904-92c7-b67ce34bb084 1 3e6d114a-5084-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27813 RAZ_Carvalho_Raquel_Negrao_2014.pdf RAZ_Carvalho_Raquel_Negrao_2014.pdf 1 DB5A991CD2D7BDFC0047E3CB060855C8 f6c462645c6aaa508afcfc35b23c1a6506f28d87a501edf3a173c7e586b444da c03b873c-5084-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27814 https://doi.org/10.1093/toxsci/kfu118 0 cf3309d5-5084-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27815 Nacionalni inštitut za biologijo 0 0 0