20.500.12556/DiRROS-19997 Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato The purpose of the study was to investigate the role of salicylic acid (SA) signalling in Ny-1-mediated hypersensitive resistance (HR) of potato (Solanum tuberosum L.) to Potato virus Y (PVY). The responses of the Ny-1 allele in the Rywal potato cultivar and transgenic NahG-Rywal potato plants that do not accumulate SA were characterized at the cytological, biochemical, transcriptome, and proteome levels. Analysis of noninoculated and inoculated leaves revealed that HR lesions started to develop from 3 d post inoculation and completely restricted the virus spread. At the cytological level, features of programmed cell death in combination with reactive oxygen species burst were observed. In response to PVY infection, SA was synthesized de novo. The lack of SA accumulation in the NahG plants led to the disease phenotype due to unrestricted viral spreading. Grafting experiments show that SA has a critical role in the inhibition of PVY spreading in parenchymal tissue, but not in vascular veins. The whole transcriptome analysis confirmed the central role of SA in orchestrating Ny-1-mediated responses and showed that the absence of SA leads to significant changes at the transcriptome level, including a delay in activation of expression of genes known to participate in defence responses. Moreover, perturbations in the expression of hormonal signalling genes were detected, shown as a switch from SA to jasmonic acid/ethylene signalling. Viral multiplication in the NahG plants was accompanied by downregulation of photosynthesis genes and activation of multiple energy-producing pathways. plant-pathogen interactions Potato virus Y salicylic acid whole transcriptome analysis Potyviridae Solanum tuberosum true false true Angleški jezik Latinski jezik Neznano 2024-08-01 15:11:51 2024-08-01 15:11:51 2024-08-02 03:50:43 0000-00-00 00:00:00 2014 0 0 str. 1095-1109 iss. 4 Vol. 65 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2014-03-01 578 0022-0957 10.1093/jxb/ert447 3027535 RAZ_Baebler_Spela_2014.pdf RAZ_Baebler_Spela_2014.pdf 1 0EC015FDFB4E88E185EAFD73F8C7FA8F e1d948ca8b7e6684571c2e88490ffe5f38dd5cae60b3d75a54a6b5430ea76180 94e7831a-4ff7-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27796 http://dx.doi.org/10.1093/jxb/ert447 0 ae58ff0e-4ff7-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27797 Nacionalni inštitut za biologijo 0 0 0