20.500.12556/DiRROS-19966 The titre of the virus in the inoculum affects the titre of the viral RNA in the host plant and the occurrence of the disease symptoms Potato virus Y (PVY) is the most economically important potato virus, therefore extensive research is focusing on elucidation of its interaction with the host. To obtain repeatable results, strict standardization of research methods is crucial. Mechanical inoculation by rubbing sap from a PVY infected plant onto the leaf surface together with a fine abrasive powder is the most convenient way of experimental transmission of PVY to host plants. However, factors determining reproducibility of this process need to be determined. In the present study, it was shown that higher titre of the virus in the inoculum resulted in faster increase of PVYNTN RNA titre in the inoculated leaves, as well as in faster translocation of PVYNTN from inoculated leaves into upper non-inoculated leaves. The final titre of PVYNTN RNA in upper non-inoculated leaves was independent of the virus titre in the inoculum. In addition, the occurrence of the disease symptoms was followed and the dependence to the titre of the virus in the inoculum was observed. krompir virus krompirja Y mehanska inokulacija titer virusa inokulum Potato virus Y PVY potato mechanical inoculation inoculum virus titre symptoms true false true Angleški jezik Slovenski jezik Neznano 2024-07-31 14:33:01 2024-07-31 14:33:01 2024-08-02 03:39:54 0000-00-00 00:00:00 2019 0 0 Nasl. z nasl. zaslona; Opis vira z dne 29. 11. 2018; str. 45-49 no. 1 Vol. 66 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-02-15 577.083.2:582.930.11 1580-3155 10.17344/acsi.2019.4585 4899407 https://journals.matheo.si/index.php/ACSi/article/viewFile/4585/1912 1 4e1288ac-4f28-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27721 RAZ_Pompe_Novak_Marusa_2019.pdf RAZ_Pompe_Novak_Marusa_2019.pdf 1 796DBC60B0FA8FB8B80CF2863E9B03E6 8c0485f9519b46dd2d765cc21ec739b23b2fc239c31efd9b19bbecd4c8168e96 93b82d77-4f28-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27722 https://www.dlib.si/details/URN:NBN:SI:doc-MGXLEA5W 0 a76cd164-4f28-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27723 Nacionalni inštitut za biologijo 0 0 0