20.500.12556/DiRROS-19891 The association of genetic factors with serum calretinin levels in asbestos-related diseases Background. Asbestos exposure is associated with different asbestos-related diseases, including malignant meso-thelioma (MM). MM diagnosis is confirmed with immunohistochemical analysis of several markers, including calretinin. Increased circulating calretinin was also observed in MM. The aim of the study was to determine if CALB2 polymor-phisms or polymorphisms in genes that can regulate calretinin expression are associated with serum calretinin levels or MM susceptibility.Subjects and methods. The study included 288 MM patients and 616 occupationally asbestos-exposed subjects without MM (153 with asbestosis, 380 with pleural plaques and 83 without asbestos-related disease). Subjects were genotyped for seven polymorphisms in CALB2, E2F2, MIR335, NRF1 and SEPTIN7 genes using competitive allele-specific polymerase chain reaction (PCR). Serum calretinin was determined with ELISA in 545 subjects. Nonparametric tests, logistic regression and receiver operating characteristic (ROC) curve analysis were used for statistical analysis.Results. Carriers of at least one polymorphic CALB2 rs889704 allele had lower calretinin levels (P = 0.036). Carriers of two polymorphic MIR335 rs3807348 alleles had higher calretinin (P = 0.027), while carriers of at least one polymorphic NRF1 rs13241028 allele had lower calretinin levels (P = 0.034) in subjects without MM. Carriers of two polymorphic E2F2rs2075995 alleles were less likely to develop MM (odds ratio [OR] = 0.64, 95% confidence interval [CI] = 0.43-0.96, P = 0.032), but the association was no longer significant after adjustment for age (P = 0.093). Optimal serum calretinin cut-off values differentiating MM patients from other subjects differed according to CALB2, NRF1, E2F2, and MIR335genotypes.Conclusions. The results of presented study suggest that genetic variability could influence serum calretinin levels. These findings could contribute to a better understanding of calretinin regulation and potentially to earlier MM diag-nosis. malignant mesothelioma calretinin CALB2 asbestos-related disease polymorphism maligni mezoteliom kalretinin bolezni povezane z azbestom polimorfizem true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-07-26 10:52:44 2024-07-26 10:52:45 2024-07-30 03:51:10 0000-00-00 00:00:00 2023 0 0 Soavtorji: Alenka Franko, Danijela Strbac, Viljem Kovac, Vita Dolzan, Katja Goricar; str. 473-486 no. 4 Vol. 57 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-01-01 616-006 1318-2099 10.2478/raon-2023-0061 178900227 https://www.radioloncol.com/index.php/ro/article/view/4214/5194 1 ab2a5392-4b1b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27589 RAZ_Zupanc_Cita_2023.pdf RAZ_Zupanc_Cita_2023.pdf 1 CBB7DFB4E04B7C5097DB178825E773B9 9ae1250f1f7bec66536e0cd91e2a3c11d79a291d3a1e3f5f6e2172d4bc8389e0 f6e2fbff-4b1b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27590 Onkološki inštitut Ljubljana 0 0 0