20.500.12556/DiRROS-19816 High-quality genome sequence of the radioresistant bacterium Deinococcus ficus KS 0460 The genetic platforms of Deinococcus species remain the only systems in which massive ionizing radiation (IR)-induced genome damage can be investigated in vivo at exposures commensurate with cellular survival. We report the whole genome sequence of the extremely IR-resistant rod-shaped bacterium Deinococcus ficus KS 0460 and its phenotypic characterization. Deinococcus ficus KS 0460 has been studied since 1987, first under the name Deinobacter grandis, then Deinococcus grandis. The D. ficus KS 0460 genome consists of a 4.019 Mbp sequence (69.7% GC content and 3894 predicted genes) divided into six genome partitions, five of which are confirmed to be circular. Circularity was determined manually by mate pair linkage. Approximately 76% of the predicted proteins contained identifiable Pfam domains and 72% were assigned to COGs. Of all D. ficus KS 0460 proteins, 79% and 70% had homologues in Deinococcus radiodurans ATCC BAA-816 and Deinococcus geothermalis DSM 11300, respectively. The most striking differences between D. ficus KS 0460 and D. radiodurans BAA-816 identified by the comparison of the KEGG pathways were as follows: (i) D. ficus lacks nine enzymes of purine degradation present in D. radiodurans, and (ii) D. ficus contains eight enzymes involved in nitrogen metabolism, including nitrate and nitrite reductases, that D. radiodurans lacks. Moreover, genes previously considered to be important to IR resistance are missing in D. ficus KS 0460, namely, for the Mn-transporter nramp, and proteins DdrF, DdrJ and DdrK, all of which are also missing in Deinococcus deserti. Otherwise, D. ficus KS 0460 exemplifies the Deinococcus lineage. Deinococcus-Thermus Deinococcaceae Deinococcus ficus radiation-resistant rod-shaped phenotype characterization genome analysis phylogenetic analysis genomika radiorezistenca gama sevanje deinokokus true false true Angleški jezik Slovenski jezik Znanstveno delo 2024-07-25 11:35:49 2024-07-25 11:35:49 2024-07-27 03:41:06 0000-00-00 00:00:00 2017 0 0 Nasl. iz nasl. zaslona; Skupno št. avtorjev: 38; Opis vira z dne 7. 8. 2017; 11 str. Vol. 12 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2017-07-28 630*1 1944-3277 10.1186/s40793-017-0258-y 4848806 http://dx.doi.org/10.1186/s40793-017-0258-y 1 89a8cd29-4a58-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27468 RAZ_Matrosova_Vera_Y._2017.pdf RAZ_Matrosova_Vera_Y._2017.pdf 1 78311B6D80EA8A62D020F74599106404 350ad0196609a2f88f486d7b0532de1341c38f450a6b50621ce7eb510fb27a91 83bd79c1-4a59-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27473 Nacionalni inštitut za biologijo 0 0 0