20.500.12556/DiRROS-19791 Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenolsand polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and inter-fere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabo-lism, while based on our recently published one for quantitative measurement of activities using coupled spectrophoto-metric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grape-vine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteriašCandidatusPhytoplasma solani’, known to alter carbohydrate metabolism in grapevine. The described adaptations maybe useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inhe-rently high levels of phenolic compounds. AGPase carbohydrates invertases sucrose synthase panel of enzyme activity assays phytoplasma true false true Angleški jezik Slovenski jezik Neznano 2024-07-25 04:14:12 2024-07-25 18:01:13 2024-07-26 04:28:28 0000-00-00 00:00:00 2016 0 0 str. 757-762 no. 4 Vol. 63 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2016-09-23 577 1318-0207 4151631 https://journals.matheo.si/index.php/ACSi/article/view/2484/1115 1 d1876eb2-4a1a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27407 RAZ_Dunn_Covington_Elizabeth_2016.pdf RAZ_Dunn_Covington_Elizabeth_2016.pdf 1 59791CF212E7AD03EED7752B8DA8DC13 0799afbc3e6d89175fd1c28b1467121b334b8976f5dc3391c824118247e614eb a0fbb00c-4a8e-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27554 Nacionalni inštitut za biologijo 0 0 0