20.500.12556/DiRROS-19781 LAMP assay and rapid sample preparation method for on-site detection of flavescence dorée phytoplasma in grapevine In Europe the most devastating phytoplasma associated with grapevine yellows (GY) diseases is a quarantine pest, flavescence dorée (FDp), from the 16SrV taxonomic group. The on-site detection of FDp with an affordable device would contribute to faster and more efficient decisions on the control measures for FDp. Therefore, a real-time isothermal LAMP assay for detection of FDp was validated according to the EPPO standards and MIQE guidelines. The LAMP assay was shown to be specific and extremely sensitive, because it detected FDp in all leaf samples that were determined to be FDp infected using quantitative real-time PCR. The whole procedure of sample preparation and testing was designed and optimized for on-site detection and can be completed in one hour. The homogenization procedure of the grapevine samples (leaf vein, flower or berry) was optimized to allow direct testing of crude homogenates with the LAMP assay, without the need for DNA extraction, and was shown to be extremely sensitive. flavescence dorée homogenization loop-mediated isothermal amplification on-site application validation true false true Angleški jezik Ni določen Neznano 2024-07-25 04:12:50 2024-07-26 13:25:52 2024-07-27 04:00:54 0000-00-00 00:00:00 2015 0 0 str. 286-296 no. 2 Vol. 64 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2015-04-01 577.2 0032-0862 10.1111/ppa.12266 3175247 http://onlinelibrary.wiley.com/doi/10.1111/ppa.12266/abstract;jsessionid=7850B082A5DCFD15057B22A9A431AFE1.f03t02 1 a2608237-4a1a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27402 RAZ_Kogovsek_Polona_2015.pdf RAZ_Kogovsek_Polona_2015.pdf 1 658F0BDBF5F2A1BEA44A1431455F8AD8 ed02c725bff9231d8582cc25495d39fd96b8eabfbf4a53a63f20b6299f39c0ac 5b169728-4b31-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27626 https://doi.org/10.1111/ppa.12266 0 4a77da27-4b31-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27625 Nacionalni inštitut za biologijo 0 0 0