20.500.12556/DiRROS-19751 Localization patterns of cathepsins K and X and their predictive value in glioblastoma Background Glioblastoma is a highly aggressive central nervous system neoplasm characterized by extensive infiltration of malignant cells into brain parenchyma, thus preventing complete tumor eradication. Cysteine cathepsins B, S, L and K are involved in cancer progression and are overexpressed in glioblastoma. We report here for the first time that cathepsin X mRNA and protein are also abundantly present in malignant glioma. Materials and methods Gene expression of cathepsins K and X was analyzed using publically-available tran-scriptomic datasets and correlated with glioma grade and glioblastoma subtype. Kaplan-Maier survival analysis was performed to evaluate the predictive value of cathepsin K and X mRNA expression. Cathepsin protein expression was localized and semi-quantified in tumor tissues by immunohistochemistry. Results Highest gene expression of cathepsins K and X was found in glioblastoma, in particular in the mesenchymal subtype. Overall, high mRNA expression of cathepsin X, but not that of cathepsin K, correlated with poor patients’ survival. Cathepsin K and X proteins were abundantly and heterogeneously expressed in glioblastoma tissue. Immuno-labeling of cathepsins K and X was observed in areas of CD133-positive glioblastoma stem cells, localized around arterioles in their niches that also expressed SDF-1α and CD68. mRNA levels of both cathepsins K and X correlated with mRNA levels of markers of glioblastoma stem cells and their niches. Conclusions The presence of both cathepsins in glioblastoma stem cell niche regions indicates their possible role in regulation of glioblastoma stem cell homing in their niches. The clinical relevance of this data needs to be elaborated in further prospective studies. cathepsins glioblastoma immunohistochemistry patient survival cancer stem cell niches true false true Angleški jezik Slovenski jezik Neznano 2024-07-24 13:28:19 2024-07-24 13:28:19 2024-07-26 03:49:29 0000-00-00 00:00:00 2018 0 0 str. 433-442, VI no. 4 Vol. 52 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2018-12-01 577.2 1318-2099 10.2478/raon-2018-0040 4873295 RAZ_Breznik_Barbara_2018.pdf RAZ_Breznik_Barbara_2018.pdf 1 8602B867411F52C64FECAB367B26CD69 c8dadf864f13c336b29e841ae514e0654897c2d2ec717cb7db0eaa4e08d5cf63 4d99060d-499f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27328 https://doi.org/10.2478/raon-2018-0040 0 648dc98f-499f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27329 Nacionalni inštitut za biologijo 0 0 0