20.500.12556/DiRROS-19723 Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysis. With the rapidly growing number of GMOs on the world market, qPCR analysis becomes laborious and expensive. Innovative cost-effective approaches are therefore urgently needed. Here, we report the development and inter-laboratory assessment of multiplex assays to quantify GMO soybean using droplet digital PCR (ddPCR). The assays were developed to facilitate testing of foods and feed for compliance with current GMO regulations in the European Union (EU). Within the EU, the threshold for labelling is 0.9% for authorised GMOs per ingredient. Furthermore, the EU has set a technical zero tolerance limit of 0.1% for certain unauthorised GMOs. The novel multiplex ddPCR assays developed target 11 GMO soybean lines that are currently authorised, and four that are tolerated, pending authorisation in the EU. Potential significant improvements in cost efficiency are demonstrated. Performance was assessed for the critical parameters, including limits of detection and quantification, and trueness, repeatability, and robustness. Inter-laboratory performance was also determined on a number of proficiency programme and real-life samples. droplet digital PCR genetically modified organisms soybean true false true Angleški jezik Ni določen Neznano 2024-07-24 03:47:37 2024-07-25 09:33:40 2024-07-26 04:28:22 0000-00-00 00:00:00 2017 0 0 Nasl. z nasl. zaslona; Opis vira z dne 28. 8. 2017; str. 1-11 Vol. 7 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2017-08-17 577.2 2045-2322 10.1038/s41598-017-09377-w 4399695 https://www.nature.com/articles/s41598-017-09377-w.pdf 1 0865cc40-494e-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27242 RAZ_Bogozalec_Kosir_Alexandra_2017.pdf RAZ_Bogozalec_Kosir_Alexandra_2017.pdf 1 32749E404B713DB980E602988272F073 82a5efc4dbd6dcf73fbf590cf4a8dc931a4e9b586cac5c5ecf72755a2ef8bb45 87d1d019-4a47-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27444 Nacionalni inštitut za biologijo 0 0 0