20.500.12556/DiRROS-19718 RNA-dependent RNA polymerase 1 in potato (Solanum tuberosum) and its relationship to other plant RNA-dependent RNA polymerases Cellular RNA-dependent RNA polymerases (RDRs) catalyze synthesis of double-stranded RNAs that can serve to initiate or amplify RNA silencing. Arabidopsis thaliana has six RDR genes; RDRs 1, 2 and 6 have roles in anti-viral RNA silencing. RDR6 is constitutively expressed but RDR1 expression is elevated following plant treatment with defensive phytohormones. RDR1 also contributes to basal virus resistance. RDR1 has been studied in several species including A. thaliana, tobacco (Nicotiana tabacum), N. benthamiana, N. attenuata and tomato (Solanum lycopersicum) but not to our knowledge in potato (S. tuberosum). StRDR1 was identified and shown to be salicylic acid-responsive. StRDR1 transcript accumulation decreased in transgenic potato plants constitutively expressing a hairpin construct and these plants were challenged with three viruses: potato virus Y, potato virus X, and tobacco mosaic virus. Suppression of StRDR1 gene expression did not increase the susceptibility of potato to these viruses. Phylogenetic analysis of RDR genes present in potato and in a range of other plant species identified a new RDR gene family, not present in potato and found only in Rosids (but apparently lost in the Rosid A. thaliana) for which we propose the name RDR7. RNA polymerases RNA potato Solanum tuberosum true false true Angleški jezik Latinski jezik Neznano 2024-07-24 03:47:11 2024-07-25 17:17:57 2024-07-26 04:28:21 0000-00-00 00:00:00 2016 0 0 Nasl. z nasl. zaslona; Opis vira z dne 17. 3. 2016; str. 1-11 Vol. 6 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2016-03-16 577.2 2045-2322 10.1038/srep23082 3814479 http://dx.doi.org/10.1038/srep23082 1 f8672fdb-494d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27239 RAZ_Hunter_Lydia_J._R._2016.pdf RAZ_Hunter_Lydia_J._R._2016.pdf 1 C660D1C9B0FED9F88EA2B370AA70AB83 35814c000a103f8ca80ed8e7fcfd5aece21211d3fd1a09c7d722db78802ba9eb 62867e0d-4a88-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27537 Nacionalni inštitut za biologijo 0 0 0