20.500.12556/DiRROS-19709 Improved protective effect of umbilical cord stem cell transplantation on cisplatin-induced kidney injury in mice pretreated with antithymocyte globulin Mesenchymal stem cells (MSCs) are recognised as a promising tool to improve renal recovery in experimental models of cisplatin-induced acute kidney injury. However, these preclinical studies were performed on severely immunodeficient animals. Here, we investigated whether human umbilical cord derived MSC treatment could equally ameliorate acute kidney injury induced by cisplatin and prolong survival in mice with a normal immune system and those with a suppressed immune system by polyclonal antithymocyte globulin (ATG). We demonstrated that ATG pretreatment, when followed by MSC transplantation, significantly improved injured renal function parameters, as evidenced by decreased blood urea nitrogen and serum creatinine concentration, as well as improved renal morphology. This tissue restoration was also supported by increased survival of mice. The beneficial effects of ATG were associated with reduced level of inflammatory protein serum amyloid A3 and induced antioxidative expression of superoxide dismutase-1 (SOD-1), glutathione peroxidase (GPx), and hem oxygenase-1 (HO-1). Infused MSCs became localised predominantly in peritubular areas and acted to reduce renal cell death. In conclusion, these results show that ATG diminished in situ inflammation and oxidative stress associated with cisplatin-induced acute kidney injury, the effects that may provide more favourable microenvironment for MSC action, with consequential synergistic improvements in renal injury and animal survival as compared to MSC treatment alone. mesenchymal stem cells nephrotoxicity true false true Angleški jezik Ni določen Neznano 2024-07-24 03:46:33 2024-07-25 17:13:47 2024-07-26 04:28:16 0000-00-00 00:00:00 2016 0 0 Nasl. z nasl. zaslona; Opis vira z dne 7. 1. 2016; str. 1-12 Vol. 2016 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2016-06-01 577.2 1687-9678 10.1155/2016/3585362 3713871 http://dx.doi.org/10.1155/2016/3585362 1 e1d3f885-494d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27234 RAZ_Veceric-Haler_Zeljka_2016.pdf RAZ_Veceric-Haler_Zeljka_2016.pdf 1 4B034231D577409C86A19ED670B93287 3260445602ba9ef00c526953ca16d870488f8be24df8beb0179905a096e062b3 fba29237-4a87-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27535 Nacionalni inštitut za biologijo Onkološki inštitut Ljubljana 0 0 0