20.500.12556/DiRROS-19691 Primary metabolism, phenylpropanoids and antioxidant pathways are regulated in potato as a response to Potato virus Y infection Potato production is one of the most important agricultural sectors, and it is challenged by various detrimental factors, including virus infections. To control losses in potato production, knowledge about the virus—plant interactions is crucial. Here, we investigated the molecular processes in potato plants as a result of Potato virus Y (PVY) infection, the most economically important potato viral pathogen. We performed an integrative study that links changes in the metabolome and gene expression in potato leaves inoculated with the mild PVYN and aggressive PVYNTN isolates, for different times through disease development. At the beginning of infection (1 day post-inoculation), virus-infected plants showed an initial decrease in the concentrations of metabolites connected to sugar and amino-acid metabolism, the TCA cycle, the GABA shunt, ROS scavangers, and phenylpropanoids, relative to the control plants. A pronounced increase in those metabolites was detected at the start of the strong viral multiplication in infected leaves. The alterations in these metabolic pathways were also seen at the gene expression level, as analysed by quantitative PCR. In addition, the systemic response in the metabolome to PVY infection was analysed. Systemic leaves showed a less-pronounced response with fewer metabolites altered, while phenylpropanoid-associated metabolites were strongly accumulated. There was a more rapid onset of accumulation of ROS scavengers in leaves inoculated with PVYN than those inoculated with PVYNTN. This appears to be related to the lower damage observed for leaves of potato infected with the milder PVYN strain, and at least partially explains the differences between the phenotypes observed. molekularna biologija true false true Angleški jezik Slovenski jezik Neznano 2024-07-24 03:45:05 2024-07-25 17:16:26 2024-07-26 04:28:07 0000-00-00 00:00:00 2016 0 0 Nasl. z nasl. zaslona; Opis vira z dne 12. 1. 2016; str. 1-20 no. 1 Vol. 11 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2016-01-04 577 1932-6203 10.1371/journal.pone.0146135 3717967 http://dx.doi.org/10.1371/journal.pone.0146135 1 acc6ffca-494d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27226 RAZ_Kogovsek_Polona_2016.pdf RAZ_Kogovsek_Polona_2016.pdf 1 64DE4238E12CBF75BA648F56352C136F 40d2e2d75b9f5235f8bd0aa00c0d31304b6dd0abf74ffea9df1d81f986a3d8e4 30d9b1e5-4a88-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27536 Nacionalni inštitut za biologijo 0 0 0