20.500.12556/DiRROS-19686 Comparison between proteome and transcriptome response in potato (Solanum tuberosum L.) leaves following potato virus Y (PVY) infection Plant diseases caused by viral infection are affecting all major crops. Being an obligate intracellular organisms, chemical control of these pathogens is so far not applied in the field except to control the insect vectors of the viruses. Understanding of molecular responses of plant immunity is therefore economically important, guiding the enforcement of crop resistance. To disentangle complex regulatory mechanisms of the plant immune responses, understanding system as a whole is a must. However, integrating data from different molecular analysis (transcriptomics, proteomics, metabolomics, smallRNA regulation etc.) is not straightforward. We evaluated the response of potato (Solanum tuberosum L.) following the infection with potato virus Y (PVY). The response has been analyzed on two molecular levels, with microarray transcriptome analysis and mass spectroscopy-based proteomics. Within this report, we performed detailed analysis of the results on both levels and compared two different approaches for analysis of proteomic data (spectral count versus MaxQuant). To link the data on different molecular levels, each protein was mapped to the corresponding potato transcript according to StNIB paralogue grouping. Only 33% of the proteins mapped to microarray probes in a one-to-one relation and additionally many showed discordance in detected levels of proteins with corresponding transcripts. We discussed functional importance of true biological differences between both levels and showed that the reason for the discordance between transcript and protein abundance lies partly in complexity and structure of biological regulation of proteome and transcriptome and partly in technical issues contributing to it. proteomics transcriptomics potato potato virus Y Solanum tuberosum true false true Angleški jezik Latinski jezik Neznano 2024-07-24 03:44:44 2024-07-24 15:49:44 2024-07-25 04:14:55 0000-00-00 00:00:00 2017 0 0 Nasl. z nasl. zaslona; Opis vira z dne 7. 7. 2017; str. 1-12 issue 3 Vol. 5 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2017-07-06 578.2 2227-7382 10.3390/proteomes5030014 4368463 http://www.mdpi.com/2227-7382/5/3/14 1 a0abfe3a-494d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27224 RAZ_Stare_Tjasa_2017.pdf RAZ_Stare_Tjasa_2017.pdf 1 6AE9F4A5C2EEFA74F107C07363CF9EEF b402b10a92dbc9b929510d765724f9684418ac1af9f0fbaef91cc1ae115ce855 dc203623-49b2-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27396 Nacionalni inštitut za biologijo 0 0 0