20.500.12556/DiRROS-19641 DNA metabarcoding uncovers fungal diversity of mixed airborne samples in Italy Fungal spores and mycelium fragments are particles which become and remain airborne and have been subjects of aerobiological studies. The presence and the abundance of taxa in aerobiological samples can be very variable and impaired by changeable climatic conditions. Because many fungi produce mycotoxins and both their mycelium fragments and spores are potential allergens, monitoring the presence of these taxa is of key importance. So far data on exposure and sensitization to fungal allergens are mainly based on the assessment of few, easily identifiable taxa and focused only on certain environments. The microscopic method used to analyze aerobiological samples and the inconspicuous fungal characters do not allow a in depth taxonomical identification. Here, we present a first assessment of fungal diversity from airborne samples using a DNA metabarcoding analysis. The nuclear ITS2 region was selected as barcode to catch fungal diversity in mixed airborne samples gathered during two weeks in four sites of North-Eastern and Central Italy. We assessed the taxonomic composition and diversity within and among the sampled sites and compared the molecular data with those obtained by traditional microscopy. The molecular analyses provide a tenfold more comprehensive determination of the taxa than the traditional morphological inspections. Our results prove that the metabarcoding analysis is a promising approach to increases quality and sensitivity of the aerobiological monitoring. The laboratory and bioinformatic workflow implemented here is now suitable for routine, high-throughput, regional analyses of airborne fungi. fungi fungal spores taxonomy sequence databases true false true Angleški jezik Ni določen Neznano 2024-07-23 03:54:25 2024-07-24 12:52:22 2024-07-25 04:14:50 0000-00-00 00:00:00 2018 0 0 Nasl. z nasl. zaslona; Opis vira z dne 22. 3. 2018; str. 1-20 iss. 3 Vol. 13 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2018-03-20 579 1932-6203 10.1371/journal.pone.0194489 4655951 RAZ_Banchi_Elisa_2018.pdf RAZ_Banchi_Elisa_2018.pdf 1 AB2C9C9BC032DC4F0C583AFC6258CCE6 84b5c9a1212e6427f3e316f66bcc7a42e90c745f308924a4dc179559623c0ef0 80056aed-499a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27301 https://doi.org/10.1371/journal.pone.0194489 0 6903d989-499a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27300 Nacionalni inštitut za biologijo 0 0 0