20.500.12556/DiRROS-19617 Morphological features of breast cancer circulating tumor cells in blood after physical and biological type of isolation Background. Circulating tumor cells (CTCs) have become an important biomarker in breast cancer. Different iso-lation tech-niques based on their biological or physical features were established. Currently, the most widely used methods for visualization after their separation are based on immunofluorescent staining, which does not provide the information on the morphology.Materials and methods. The aim of this study was to evaluate how two different separation techniques affect cell morphology and to analyse cell morphology with techniques used in routine cytopathological laboratory. A direct side-by-side comparison of physical (Parsortix%) and biological (MACS%) separation technique was performed.Results. In the preclinical setting, both isolation techniques retained the viability and antigenic characteristics of MCF7 breast cancer cells. Some signs of degeneration such as cell swelling, cytoplasmic blebs, villous projections and vacuolization were observed. In metastatic breast cancer patient cohort, morphological features of isolated CTCs were dependent on the separation technique. After physical separation, CTCs with preserved cell morphology were detected. After biological separation the majority of the isolated CTCs were so degenerated that their identity was difficult to confirm.Conclusions. Taken together, physical separation is a suitable technique for detection of CTCs with preserved cell morphology for the use in a routine cytopathological laboratory. circulating tumor cells breast cancer morphology CTC rak dojke morfologija true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-07-22 14:46:43 2024-07-22 14:46:43 2024-07-23 03:55:56 0000-00-00 00:00:00 2021 0 0 str. 292-304, VI no. 3 Vol. 55 Sep. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-09-01 602 1318-2099 10.2478/raon-2021-0033 75204867 https://www.sciendo.com/article/10.2478/raon-2021-0033 1 afeb38ea-4817-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27116 RAZ_Jesenko_Tanja_2021.pdf RAZ_Jesenko_Tanja_2021.pdf 1 5A686366820E14A725A87B83614E6EDA e42cfec26c4705bed43f415db7ce9cabe993ff04c80a8575206a599c565f439c dc1f596e-4817-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27117 Onkološki inštitut Ljubljana 0 0 0