20.500.12556/DiRROS-19599 Rapid molecular detection and transmission of bacterial leaf streak pathogen, Xanthomonas oryzae pv. oryzicola, in rice seeds Xanthomonas oryzae pv. oryzicola (Xoc) is a seed-borne bacterial pathogen of rice. In this study, a rapid molecular detection method in seeds was developed to prevent unwanted movement of infected materials and greater yield loss. Crude DNA from ground seed extract supernatant was precipitated in ethanol and centrifuged. DNA pellets of homogenous subsamples were pooled and werepurified using SDS or a DNA extraction kit. Three pairs of primers previously designed on Xoc isolate BLS-256 genome sequence robustly amplify Xoc-specific DNA sequences. Primer T40 was found to efficiently detect Xoc but only in single primer reactions. While primers 3864 and 3866 can amplify Xoc DNA separately, however, their multiplex PCR reactions were unsuccessful, probably due to substrate competition in the reaction mixture. Optimized PCR mixture and conditions were able to detect as low as 10 cfu/mL Xoc cells and the direct extraction method and conditions were able to detect as low as 104 cfu/mL inoculated cells using primer T40. Lastly, Xoc transmission through seeds was observed only in plant samples which were heavily infected. Recovery from infection, at around 25-50% was also observed in some cultivars which also manifested good grain filling at 56 days after transplanting. biotehnologija rastline molekularna diagnostika patogeni semena true false true Angleški jezik Slovenski jezik Neznano 2024-07-20 04:02:51 2024-07-24 10:35:10 2024-07-25 04:14:47 0000-00-00 00:00:00 2019 0 0 Nasl. z nasl. zaslona; Opis vira z dne 16. 4. 2021; Besedilo v PDF obsega 10 str.; str. 509-520 Vol. 16 iss. 3 Sept. 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-09-22 60 2456-2602 10.13005/bbra/2765 59917571 http://www.biotech-asia.org/vol16no3/rapid-molecular-detection-and-transmission-of-bacterial-leaf-streak-pathogen-xanthomonas-oryzae-pv-oryzicola-in-rice-seeds/ 1 721c8ccf-462b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27050 RAZ_Rivarez_Mark_Paul_Selda_2019.pdf RAZ_Rivarez_Mark_Paul_Selda_2019.pdf 1 F800399F48F352F1C0E952B041CC0217 b71866a213ee2cb6aa58c7cdb88959e97b1eccf5cb69a07124690ec1080ee2dd 0b243f9c-4987-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27271 Nacionalni inštitut za biologijo 0 0 0