20.500.12556/DiRROS-19597 Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice Insight into the molecular and cellular processes in blood-retinal barrier (BRB) development, including the contribution of paracellular and transcellular pathways, is still incomplete but may help to understand the inverse process of BRB loss in pathologic eye conditions. In this comprehensive observational study, we describe in detail the formation of the BRB at the molecular level in physiologic conditions, using mice from postnatal day (P)3 to P25. Our data indicate that immature blood vessels already have tight junctions at P5, before the formation of a functional BRB. Expression of the endothelial cell-specific protein plasmalemma vesicle-associated protein (PLVAP), which is known to be involved in transcellular transport and associated with BRB permeability, decreased during development and was absent when a functional barrier was formed. Moreover, we show that PLVAP deficiency causes a transient delay in retinal vascular development and changes in mRNA expression levels of endothelial permeability pathway proteins.—Van der Wijk, A.-E., Wisniewska-Kruk, J., Vogels, I. M. C., van Veen, H. A., Ip, W. F., van der Wei, N. N., van Noorden, C. J. F., Schlingemann, R. O., Klaassen, I. Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice. tight junctions transcellular permeability VEGF signaling biokemija celična membrana plasmalemma homeostaza true false true Angleški jezik Slovenski jezik Neznano 2024-07-20 04:02:41 2024-07-24 10:20:54 2024-07-25 04:14:46 0000-00-00 00:00:00 2019 0 0 str. 5320-5333 iss. 4 Vol. 33 Apr. 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-04-01 577 0892-6638 10.1096/fj.201801499RRR 40480773 https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.201801499RRR 1 6d220cfe-462b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27048 RAZ_Wijk_Anne-Eva_van_der_2019.pdf RAZ_Wijk_Anne-Eva_van_der_2019.pdf 1 8E8F77D3868E27DD82E50EE17497A8CD c127c450f80461e4b44e6cd1a5c744b56208bb93f83d61663d5e1ea66e5c10eb 819cc475-4985-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27269 Nacionalni inštitut za biologijo 0 0 0