20.500.12556/DiRROS-19584 Structural basis for the multitasking nature of the potato virus Y coat protein Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY’s flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA–coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein’s amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications. plant pathogens potato virus Y viral RNA true false true Angleški jezik Ni določen Neznano 2024-07-20 04:01:38 2024-07-23 16:27:02 2024-07-24 03:48:00 0000-00-00 00:00:00 2019 0 0 Nasl. z nasl. zaslona; Opis vira z dne 30. 7. 2019; str. 1-13 no. 7 Vol. 5 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-07-17 577.2 2375-2548 10.1126/sciadv.aaw3808 5135183 https://advances.sciencemag.org/content/5/7/eaaw3808 1 481a9c17-462b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27039 RAZ_Kezar_Andreja_2019.pdf RAZ_Kezar_Andreja_2019.pdf 1 4C8117F2744A889CE9588E7002A748AF af8f6187ed373f02698bacc9ad25986ec9d8aba7cf5ae0ba8f73e88848233dd1 2172b262-48ef-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27220 Kemijski inštitut Nacionalni inštitut za biologijo 0 0 0