20.500.12556/DiRROS-19583 The transcriptome of Darwinʼs bark spider silk glands predicts proteins contributing to dragline silk toughness Darwin’s bark spider (Caerostris darwini) produces giant orb webs from dragline silk that can be twice as tough as other silks, making it the toughest biological material. This extreme toughness comes from increased extensibility relative to other draglines. We show C. darwini dragline-producing major ampullate (MA) glands highly express a novel silk gene transcript (MaSp4) encoding a protein that diverges markedly from closely related proteins and contains abundant proline, known to confer silk extensibility, in a unique GPGPQ amino acid motif. This suggests C. darwini evolved distinct proteins that may have increased its dragline’s toughness, enabling giant webs. Caerostris darwini’s MA spinning ducts also appear unusually long, potentially facilitating alignment of silk proteins into extremely tough fibers. Thus, a suite of novel traits from the level of genes to spinning physiology to silk biomechanics are associated with the unique ecology of Darwin’s bark spider, presenting innovative designs for engineering biomaterials. spiders true false true Angleški jezik Ni določen Neznano 2024-07-20 04:01:34 2024-07-23 16:22:21 2024-07-24 03:48:00 0000-00-00 00:00:00 2019 0 0 Nasl. z nasl. zaslona; Opis vira z dne 29. 7. 2019; str. 1-8 [Vol.] 2 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-07-25 595.44 2399-3642 10.1038/s42003-019-0496-1 5134927 https://www.nature.com/articles/s42003-019-0496-1 1 44196726-462b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27038 RAZ_Garb_Jessica_E._2019.pdf RAZ_Garb_Jessica_E._2019.pdf 1 C005D58CABA12BA729F7ED1D9DBB2D97 ee6a91b6f379fab1e39686910b13088cfb5c30972c9c1c200d906121ae174588 b2da90bd-48ee-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27219 Nacionalni inštitut za biologijo 0 0 0