20.500.12556/DiRROS-19581 Digital PCR as an effective tool for GMO quantification in complex matrices The increased use of genetically modified organisms (GMOs) is accompanied by increased complexity of the matrices that contain GMOs. The most common DNA-based approach for GMO detection and quantification is real-time quantitative polymerase chain reaction (qPCR). However, as qPCR is sensitive to inhibitors and relies on standard curves for quantification, it has limited application in GMO quantification for complex matrices. To overcome this hurdle in DNA quantification, we present droplet digital PCR (ddPCR) assays that were designed to target ‘Roundup Ready’ soybean and the soybean reference gene. Three ddPCR assays were transferred from qPCR to QX100/QX200 ddPCR platforms and characterised. Together, the fitness-for-purpose study on four real-life samples and the use of a chamber-based PCR system, showed that dPCR has great potential to improve such measurements in GMO testing and monitoring of food authenticity. genetically modified organisms digital PCR GMO quantification complex matrices true false true Angleški jezik Ni določen Neznano 2024-07-20 04:01:17 2024-07-23 15:27:39 2024-07-24 03:48:00 0000-00-00 00:00:00 2019 0 0 str. 73-78 Vol. 294 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-10-01 577.2 0308-8146 10.1016/j.foodchem.2019.05.029 5062223 RAZ_Bogozalec_Kosir_Alexandra_2019.pdf RAZ_Bogozalec_Kosir_Alexandra_2019.pdf 1 19A2756FE96F6A32AC3019AA967E2FA1 e85f324aef4b077209ed393e52f68bc2a8a94d4ccaad1367e42e5071c59266eb 1290b108-48e8-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27216 https://doi.org/10.1016/j.foodchem.2019.05.029 0 9c4b8bec-48e7-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27215 Nacionalni inštitut za biologijo 0 0 0