20.500.12556/DiRROS-19572 Microscopy of crustacean cuticle formation of a flexible extracellular matrix in moulting sea slaters Ligia pallasii Structural and functional properties of exoskeleton in moulting sea slaters Ligia pallasii from the Eastern Pacific coast were investigated with CT scanning and electron microscopy. Ultrastructure of preecdysial and postecdysial cuticular layers was described in premoult, intramoult and postmoult animals. Cuticle is a flexible extracellular matrix connected to the epidermal cells through pore channels. During premoult epicuticle and exocuticle are formed and during intramoult and postmoult endocuticular lamellae are deposited and the cuticle is progressively constructed by thickening and mineralization. Cuticle permeability, flexibility and waterproofing capacity change accordingly. Elaboration of epicuticular scales connected to an extensive network of nanotubules, establish its anti-adhesive and hydrophobic properties. Labelling with gold conjugated WGA lectins on Tokuyashu thawed cryosections exposes differences in chitin content between exocuticle and endocuticle. Histochemical staining of cuticle shows presence of acidic carbohydrates/glycoconjugates and lipoproteins in epicuticular layer. Chitin microfibrils are formed at the microvillar border of epidermal cells with abundant Golgi apparatus and secretory vesicles. Numerous spherules associated with nanotubules were observed in the ecdysial space in intramoult animals. The mineral component of the cuticle as visualized with CT scanning indicates progressive mineral resorption from the posterior to the anterior half of the body in premoult animals, its translocation from the anterior to posterior part during intramoult and its progressive deposition in the posterior and anterior exoskeleton during postmoult. Cuticle of sea slaters is a unique biocomposite and biodynamic material constantly reconstructed during frequent moults, and adapted to specific physical and biotic conditions of the high intertidal rocky zone. cuticle ultrastructure micro CT scanning moult cycle SEM TEM terrestrial isopods Oniscidea true false true Angleški jezik Latinski jezik Neznano 2024-07-20 04:00:27 2024-07-23 14:12:44 2024-07-24 03:47:58 0000-00-00 00:00:00 2019 0 0 str. 857-865 ǂiss.ǂ4 Vol. 99 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-06-01 576 0025-3154 10.1017/S0025315418001017 4930127 https://doi.org/10.1017/S0025315418001017 1 1bfd9c21-462b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27031 RAZ_Strus_Jasna_2019.pdf RAZ_Strus_Jasna_2019.pdf 1 809DB057BAE3D844525B192DAA155E02 2e387d39cf7b1fe0c255bba8418237c5f924b50d0b1afa5762b3e825af1bfc4f 897414dc-48dc-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27194 Nacionalni inštitut za biologijo 0 0 0