20.500.12556/DiRROS-19553 Natural variation in Arabidopsis thaliana rosette area unveils new genes involved in plant development Growth is a complex trait influenced by multiple genes that act at different moments during the development of an organism. This makes it difficult to spot its underlying genetic mechanisms. Since plant growth is intimately related to the effective leaf surface area (ELSA), identifying genes controlling this trait will shed light on our understanding of plant growth. To find new genes with a significant contribution to plant growth, here we used the natural variation in Arabidopsis thaliana to perform a genome-wide association study of ELSA. To do this, the projected rosette area of 710 worldwide distributed natural accessions was measured and analyzed using the genome-wide efficient mixed model association algorithm. From this analysis, ten genes were identified having SNPs with a significant association with ELSA. To validate the implication of these genes into A. thaliana growth, six of them were further studied by phenotyping knock-out mutant plants. It was observed that rem1.2, orc1a, ppd1, and mcm4 mutants showed different degrees of reduction in rosette size, thus confirming the role of these genes in plant growth. Our study identified genes already known to be involved in plant growth but also assigned this role, for the first time, to other genes. biotehnologija rastline rast genetika true false true Angleški jezik Slovenski jezik Neznano 2024-07-19 03:45:12 2024-07-23 13:01:32 2024-07-24 03:47:57 0000-00-00 00:00:00 2020 0 0 Nasl. z nasl. zaslona; Št. članka: 17600; Soavtorji: Anamarija Butković, Mark Paul Selda Rivarez, Santiago F. Elena; Opis vira z dne 15. 4. 2021; str. 1-10 Vol. 10 Oct. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-10-19 60 2045-2322 10.1038/s41598-020-74723-4 59829251 https://www.nature.com/articles/s41598-020-74723-4 1 c5fea8a9-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26952 RAZ_Brigatti_Edgardo_2020.pdf RAZ_Brigatti_Edgardo_2020.pdf 1 B383D695372B82FB1C013AA40504B2D3 91e2388d01be322d7ae23f89fcd30ec36f421ec5bd478e6ada24e99905709df5 c57ff96f-48d2-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27184 Nacionalni inštitut za biologijo 0 0 0