20.500.12556/DiRROS-19546 Plastics in cyanobacterial blooms - genotoxic effects of binary mixtures of cylindrospermopsin and bisphenols in HepG2 cells Ever-expanding environmental pollution is causing a rise in cyanobacterial blooms and the accumulation of plastics in water bodies. Consequently, exposure to mixtures of cyanotoxins and plastic-related contaminants such as bisphenols (BPs) is of increasing concern. The present study describes genotoxic effects induced by co-exposure to one of the emerging cyanotoxins—cylindrospermopsin (CYN)—(0.5 µg/mL) and BPs (bisphenol A (BPA), S (BPS), and F (BPF); (10 µg/mL)) in HepG2 cells after 24 and 72 h of exposure. The cytotoxicity was evaluated with an MTS assay and genotoxicity was assessed through the measurement of the induction of DNA double strand breaks (DSB) with the γH2AX assay. The deregulation of selected genes (xenobiotic metabolic enzyme genes, DNA damage, and oxidative response genes) was assessed using qPCR. The results showed a moderate reduction of cell viability and induction of DSBs after 72 h of exposure to the CYN/BPs mixtures and CYN alone. None of the BPs alone reduced cell viability or induced DSBs. No significant difference was observed between CYN and CYN/BPs exposed cells, except with CYN/BPA, where the antagonistic activity of BPA against CYN was indicated. The deregulation of some of the tested genes (CYP1A1, CDKN1A, GADD45A, and GCLC) was more pronounced after exposure to the CYN/BPs mixtures compared to single compounds, suggesting additive or synergistic action. The present study confirms the importance of co-exposure studies, as our results show pollutant mixtures to induce effects different from those confirmed for single compounds. cylindrospermopsin CYN bisphenols BPA BPS BPF BPAF co-exposure genotoxicity cytotoxicity cylindrospermopsin bisfenol ko-izpostavitev genotoksičnost citotoksičnost true false true Angleški jezik Slovenski jezik Neznano 2024-07-19 03:44:51 2024-07-23 12:23:20 2024-07-24 03:47:57 0000-00-00 00:00:00 2020 0 0 Nasl. z nasl. zaslona; Opis vira z dne 1. 4. 2020; str. 1-19 iss. 4 Vol. 12 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-03-21 615.9 2072-6651 40541189 https://www.mdpi.com/2072-6651/12/4/219 1 b9535b3d-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26946 RAZ_Hercog_Klara_2020.pdf RAZ_Hercog_Klara_2020.pdf 1 49FF5ED82B374A122EF462613B7665F7 2f07581faa479927f4c43cccc7750c340ef4324e68599d2a456b5afdcbebc5d2 0c65163e-48cd-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27176 Nacionalni inštitut za biologijo 0 0 0