20.500.12556/DiRROS-19541 Characterization of In vitro 3D cell model developed from human hepatocellular carcinoma (HepG2) cell line In genetic toxicology, there is a trend against the increased use of in vivo models as highlighted by the 3R strategy, thus encouraging the development and implementation of alternative models. Two-dimensional (2D) hepatic cell models, which are generally used for studying the adverse effects of chemicals and consumer products, are prone to giving misleading results. On the other hand, newly developed hepatic three-dimensional (3D) cell models provide an attractive alternative, which, due to improved cell interactions and a higher level of liver-specific functions, including metabolic enzymes, reflect in vivo conditions more accurately. We developed an in vitro 3D cell model from the human hepatocellular carcinoma (HepG2) cell line. The spheroids were cultured under static conditions and characterised by monitoring their growth, morphology, and cell viability during the time of cultivation. A time-dependent suppression of cell division was observed. Cell cycle analysis showed time-dependent accumulation of cells in the G0/G1 phase. Moreover, time-dependent downregulation of proliferation markers was shown at the mRNA level. Genes encoding hepatic markers, metabolic phase I/II enzymes, were time-dependently deregulated compared to monolayers. New knowledge on the characteristics of the 3D cell model is of great importance for its further development and application in the safety assessment of chemicals, food products, and complex mixtures. in vitro 3D celični model HepG2 celična proliferacija izražanje genov true false true Angleški jezik Slovenski jezik Neznano 2024-07-19 03:44:25 2024-07-22 12:02:17 2024-07-23 03:55:54 0000-00-00 00:00:00 2020 0 0 Nasl. z nasl. zaslona; Opis vira z dne 30. 11. 2020; Soavtorji: Barbara Breznik, Metka Filipič, Bojana Žegura; Članek št. 2557; str. 1-19 iss. 12 Vol. 9 Nov. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-11-01 577 2073-4409 10.3390/cells9122557 40005123 https://www.mdpi.com/2073-4409/9/12/2557 1 a9ff1320-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26941 RAZ_Stampar_Martina_2020.pdf RAZ_Stampar_Martina_2020.pdf 1 A0B918E65D16555D4F3609F84F8035AD 51d0247e6d8dbfd6e8db3536697929a19eaba09ca79b501a8b104d1c91d49235 eb35be66-4800-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27088 Nacionalni inštitut za biologijo 0 0 0