20.500.12556/DiRROS-19530 Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato Although the reference genome of Solanum tuberosum Group Phureja double-monoploid (DM) clone is available, knowledge on the genetic diversity of the highly heterozygous tetraploid Group Tuberosum, representing most cultivated varieties, remains largely unexplored. This lack of knowledge hinders further progress in potato research. In conducted investigation, we first merged and manually curated the two existing partially-overlapping DM genome-based gene models, creating a union of genes in Phureja scaffold. Next, we compiled available and newly generated RNA-Seq datasets (cca. 1.5 billion reads) for three tetraploid potato genotypes (cultivar Désirée, cultivar Rywal, and breeding clone PW363) with diverse breeding pedigrees. Short-read transcriptomes were assembled using several de novo assemblers under different settings to test for optimal outcome. For cultivar Rywal, PacBio Iso-Seq full-length transcriptome sequencing was also performed. EvidentialGene redundancy-reducing pipeline complemented with in-house developed scripts was employed to produce accurate and complete cultivar-specific transcriptomes, as well as to attain the pan-transcriptome. The generated transcriptomes and pan-transcriptome represent a valuable resource for potato gene variability exploration, high-throughput omics analyses, and breeding programmes. RNA interferenca (RNAi) dsRNA utišanje genov RNAi za zatiranje škodljivcev analiza preživetja Leptinotarsa decemlineata (Say) poljski poskus E coli HT115 (DE3) true false true Angleški jezik Slovenski jezik Neznano 2024-07-19 03:43:42 2024-07-22 11:24:20 2024-07-23 03:55:52 0000-00-00 00:00:00 2020 0 0 Nasl. z nasl. zaslona; Opis vira z dne 9. 9. 2020; str. 1-15 article no. ǂ249 Vol. 7 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-07-24 577 2052-4463 10.1038/s41597-020-00581-4 27695619 https://www.nature.com/articles/s41597-020-00581-4 1 90e0768d-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26931 RAZ_Petek_Marko_2020.pdf RAZ_Petek_Marko_2020.pdf 1 BE72799DB1A70D9D867AB615DCB19956 d552ccdde79ee910d49860914ca73c48b9ed7f85001f7e902818a24bec78d440 bfef84e8-47fb-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27076 Nacionalni inštitut za biologijo 0 0 0