20.500.12556/DiRROS-19525 2D and 3D in vitro assays to quantify the invasive behavior of glioblastoma stem cells in response to SDF-1[alpha] Invasion is a hallmark of cancer and therefore in vitro invasion assays are important tools in cancer research. We aimed to describe in vitro 2D transwell assays and 3D spheroid assays to quantitatively determine the invasive behavior of glioblastoma stem cells in response to the chemoattractant SDF-1α. Matrigel was used as a matrix in both assays. We demonstrated quantitatively that SDF-1α increased invasive behavior of glioblastoma stem cells in both assays. We conclude that the 2D transwell invasion assay is easy to perform, fast and less complex whereas the more time-consuming 3D spheroid invasion assay is physiologically closer to the in vivo situation. 2D transwell invasion assay 3D spheroid invasion assay cancer cell cellular invasion glioblastoma stem cells true false true Angleški jezik Ni določen Neznano 2024-07-19 03:43:16 2024-07-22 11:03:06 2024-07-23 03:55:52 0000-00-00 00:00:00 2020 0 0 str. 339-346 no. 5 Vol. 69 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-09-01 577.2 0736-6205 10.2144/btn-2020-0046 26930691 http://dx.doi.org/10.2144/btn-2020-0046 1 80593734-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26928 RAZ_Hira_Vashendriya_V._V._2020.pdf RAZ_Hira_Vashendriya_V._V._2020.pdf 1 1ED87956B30889148329277B07AB1EF4 ebb27dc8eb01f093a09fa622bd057ada06ac2e5e1c4d74985a40ea1cc2fd0d6d dbb02e08-47f8-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27069 Nacionalni inštitut za biologijo 0 0 0