20.500.12556/DiRROS-19524 The effect of three polyphenols and some other anti-oxidant substances on amyloid fibril formation by human cystatin C Human cystatin C (CysC) is an amyloid forming protein involved in the hereditary cerebral amyloid angiopathy (HCCAA) that affects arteries in the brain and the peripheral nervous system. In this study we measured the influence of several substances on human CysC aggregation and amyloid fibril formation, induced at pH 4 in vitro. The effect of three polyphenols: resveratrol, quercetin and curcumin and of two antioxidants: vitamin C (VitC) and N-acetyl-L-cysteine (NAC) was explored as well as the effect of sulphoraphane (SF) and α-lipoic acid (AL). The formation of amyloid fibrils was followed by Thioflavin T (ThT) fluorescence and by transmission electron microscopy (TEM). Effects on the length of the lag phase were revealed by following the increase of ThT fluorescence intensity with time. The amount and morphology of fibrils in comparison to prefibrillar aggregates and globular oligomers were evaluated by TEM at the plateau stage of the reaction. Thermal stabilization of the CysC monomer by the small compounds was measured by differential scanning fluorimetry (DSF). NAC, VitC and SF exhibited the largest inhibitory effect on amyloid fibril growth. The effects of polyphenols were not significant, apart from resveratrol, which partly inhibited the amyloid fibril growth. true false true Angleški jezik Ni določen Neznano 2024-07-19 03:43:12 2024-07-22 10:54:47 2024-07-23 03:55:52 0000-00-00 00:00:00 2020 0 0 str. 1-9 Vol. 140 2020 0000-00-00 Zaloznikova NiDoloceno NiDoloceno 0000-00-00 0000-00-00 2020-11-01 577 0197-0186 10.1016/j.neuint.2020.104806 26233859 http://dx.doi.org/10.1016/j.neuint.2020.104806 1 7eb4aba2-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26927 RAZ_Jahic_Alma_2020.pdf RAZ_Jahic_Alma_2020.pdf 1 AE8CB27FCAB7F417731B068312F84F37 b333322aab82397749abc289eef618455d966a88e106a438911bc6127aaea22a 644eea08-47f7-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27068 Nacionalni inštitut za biologijo 0 0 0