20.500.12556/DiRROS-19523 Validating the potential of double-stranded RNA targeting Colorado potato beetle mesh gene in laboratory and field trials Colorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying of dsRNA formulations is a promising innovative technology providing highly specific and environmentally acceptable option for CPB management. We designed dsRNA to silence CPB mesh gene (dsMESH) and performed laboratory feeding trials to assess impacts on beetle survival and development. We compared the effectiveness of in vivo and in vitro produced dsRNA in a series of laboratory experiments. We additionally performed a field trial in which the efficacy of dsRNA sprayed onto potato foliage was compared to a spinosad-based insecticide. We showed that dsMESH ingestion consistently and significantly impaired larval growth and decreased larval survival in laboratory feeding experiments. In vivo produced dsRNA performed similarly as in vitro synthesized dsRNA in laboratory settings. In the field trial, dsMESH was as effective in controlling CPB larvae as a commercial spinosad insecticide, its activity was however slower. We discuss limitations and benefits of a potential dsMESH-based CPB management strategy and list some important RNAi based CPB research topics, which will have to be addressed in future. RNA interference RNA interference feeding ds RNA gene silencing RNAi pest control survival analysis field trial Leptinotarsa decemlineata E. coli krompirjev hrošč koloradski hrošč škodljvci poljski poskusi laboratorijski poskusi RNA interferenca dvovijačna RNA zatiranje škodljivcev z RNAi analiza preživetja true false true Angleški jezik Latinski jezik Neznano 2024-07-19 03:43:06 2024-07-22 10:50:48 2024-12-13 12:09:32 0000-00-00 00:00:00 2020 0 0 Nasl. iz nasl. zaslona; Opis vira z dne 18.8.2020; ǂarticle no.ǂ1250; Str. 1-9 article no.ǂ1250 Vol. 11 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-08-19 632 1664-462X 10.3389/fpls.2020.01250 25419523 RAZ_Petek_Marko_2020.pdf RAZ_Petek_Marko_2020.pdf 1 CCB4911D13648CB638D5FF2AB7176212 51b8be15556d795c8fddc850cb042bf4cf9210a491e41cc1d609588b48afcb59 163045eb-47f7-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27067 Nacionalni inštitut za biologijo Kmetijski inštitut Slovenije 0 0 0