20.500.12556/DiRROS-19522 Development and validation of a new TaqMan real-time PCR for detection of 'Candidatus phytoplasma pruni' Development and validation of a new TaqMan real-time PCR for detection of Candidatus phytoplasma pruni Phytoplasmas of the 16SrIII group are wide spread, and have a broad plant host range. Among these, ‘Candidatus phytoplasma pruni’ (‘Ca. P. pruni’; phytoplasmas of 16SrIII subgroup A) can cause serious diseases in Prunus species and ‘Ca. P. pruni’-related strains can infect other plant species, including grapevines. In this study, a new real-time PCR detection system was developed for ‘Ca. P. pruni’ using TaqMan chemistry. This test was designed to detect ‘Ca. P. pruni’, by amplifying the species-specific secY gene. In addition, a test to amplify the group-specific 16S rRNA gene region was also developed. The performances of both tests were evaluated. The test that amplifies the secY gene provided reliable and quick detection of ‘Ca. P. pruni’. Using the newly developed and validated test, ‘Ca. P. pruni’ was not found in any of the 434 field samples collected from different plants species grown in different regions of Slovenia. phytoplasma X-disease real-time PCR Prunus rastline fitoplazma bolezni PCR true false true Angleški jezik Slovenski jezik Neznano 2024-07-19 03:43:01 2024-07-22 10:49:01 2024-07-23 03:55:51 0000-00-00 00:00:00 2020 0 0 Nasl. z nasl. zaslona; Opis vira z dne 10. 8. 2020; str. 1-13 iss. 8 Vol. 9 7 Aug. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-08-07 632 2076-0817 10.3390/pathogens9080642 24707587 https://www.mdpi.com/2076-0817/9/8/642 1 77e01703-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26926 RAZ_Kogej_Zwitter_Zala_2020.pdf RAZ_Kogej_Zwitter_Zala_2020.pdf 1 276BC66C24180804B524113FCCD46039 026b72dac1c6cfe6ca3fcdcf325effd108d994afd19139fd5247e9fc797389cb 8e18fd51-47f6-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27066 Nacionalni inštitut za biologijo 0 0 0