20.500.12556/DiRROS-19513 Functional imaging of the ocular fundus using an 8-band retinal multispectral imaging system Application of functional imaging in ophthalmology requires efficient imaging techniques that can detect and quantify chromophores to visualise processes in vivo. The aim of the present study was to develop and evaluate a fast and affordable imaging system. We describe an eight-band retinal multispectral imaging (MSI) system and compare it with a hyperspectral imaging (HSI) device. Determination of blood oxygen saturation was studied as proof of principle. Reflectance of incident light is measured as 1/absorbance at different wavelengths between 440 nm and 580 nm. Both devices have incorporated optical bandpass filters in a mydriatic fundus camera. The MSI system scans the retina at eight pre-defined wavelengths specific for the spectrum of haemoglobin. The HSI system acquires a full scan from 480 to 720 nm in 5 nm steps. A simple assessment of the ratio between the absorbance peaks of oxygenated haemoglobin (HbO2) and reduced haemoglobin (HbR) was not suitable for generating validated oxygenation maps of the retina. However, a correction algorithm that compares the measured reflectance with reflectance spectra of fully oxygenated and fully deoxygenated blood allowed our MSI setup to estimate relative oxygen saturation at higher levels, but underestimated relative oxygen saturation at lower levels. The MSI device generated better quality images than the HSI device. It allows customisation with filter sets optimised for other chromophores of interest, and augmented with extrinsic contrast imaging agents, it has the potential for a wider range of ophthalmic molecular imaging applications. functional imaging multispectral imaging hyperspectral imaging oximetry oftalmologija oksimetrija funkcionalno slikanje vizualizacija procesov true false true Angleški jezik Slovenski jezik Neznano 2024-07-19 03:42:16 2024-07-22 09:23:50 2024-07-23 03:55:51 0000-00-00 00:00:00 2020 0 0 Nasl. z nasl. zaslona; Opis vira z dne 11. 5. 2020; str. 1-14 no. 2 Vol. 4 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-05-07 617.7 2410-390X 10.3390/instruments4020012 14604291 https://doi.org/10.3390/instruments4020012 1 5d6b2f78-455f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26918 RAZ_Carvalho_J._Emanuel_Ramos_de_2020.pdf RAZ_Carvalho_J._Emanuel_Ramos_de_2020.pdf 1 EBF7FFAF18FDA576804A30A87B85BB34 3a179bcc3c909127b1d0dd66c0a7a811509f1839cf7d8aec7c9dc0a5b4231dce bb0827e4-47ea-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27055 Nacionalni inštitut za biologijo 0 0 0