20.500.12556/DiRROS-19508 Viromics and infectivity analysis reveal the release of infective plant viruses from wastewater into the environment Viruses represent one of the most important threats to agriculture. Several viral families include highly stable pathogens, which remain infective and can be transported long distances in water. The diversity of plant viruses in wastewater remains understudied; however, their potential impact is increasing with the increased irrigation usage of reclaimed wastewater. To determine the abundance, diversity and biological relevance of plant viruses in wastewater influents and effluents we applied an optimized virus concentration method followed by high-throughput sequencing and infectivity assays. We detected representatives of 47 plant virus species, including emerging crop threats. We also demonstrated infectivity for pathogenic and economically relevant plant viruses from the genus Tobamovirus (family Virgaviridae), which remain infective even after conventional wastewater treatment. These results demonstrate the potential of metagenomics to capture the diversity of plant viruses circulating in the environment and expose the potential risk of the uncontrolled use of reclaimed water for irrigation. wastewater virome high-throughput sequencing plant viruses tobamoviruses infectivity true false true Angleški jezik Ni določen Neznano 2024-07-19 03:41:55 2024-07-19 13:56:32 2024-07-20 04:03:07 0000-00-00 00:00:00 2020 0 0 str. 1-11 Vol. 177 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-06-15 577 0043-1354 10.1016/j.watres.2020.115628 5318735 RAZ_Bacnik_Katarina_2020.pdf RAZ_Bacnik_Katarina_2020.pdf 1 78120ECE84A22DBE88883FDE28C5E303 6b2a759607703330061661f4d79b7b0621c376812e1e8673942307c35ae1ad13 5af52d94-45b5-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27023 https://doi.org/10.1016/j.watres.2020.115628 0 6a2d4ef2-45b5-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27024 Nacionalni inštitut za biologijo 0 0 0