20.500.12556/DiRROS-19501 A mini-TGA protein modulates gene expression through heterogeneous association with transcription factors TGA (TGACG-binding) transcription factors, which bind their target DNA through a conserved basic region leucine zipper (bZIP) domain, are vital regulators of gene expression in salicylic acid (SA)-mediated plant immunity. Here, we investigated the role of StTGA2.1, a potato (Solanum tuberosum) TGA lacking the full bZIP, which we named a mini-TGA. Such truncated proteins have been widely assigned as loss-of-function mutants. We, however, confirmed that StTGA2.1 overexpression compensates for SA-deficiency, indicating a distinct mechanism of action compared with model plant species. To understand the underlying mechanisms, we showed that StTGA2.1 can physically interact with StTGA2.2 and StTGA2.3, while its interaction with DNA was not detected. We investigated the changes in transcriptional regulation due to StTGA2.1 overexpression, identifying direct and indirect target genes. Using in planta transactivation assays, we confirmed that StTGA2.1 interacts with StTGA2.3 to activate StPRX07, a member of class III peroxidases (StPRX), which are known to play role in immune response. Finally, via structural modeling and molecular dynamics simulations, we hypothesized that the compact molecular architecture of StTGA2.1 distorts DNA conformation upon heterodimer binding to enable transcriptional activation. This study demonstrates how protein truncation can lead to distinct functions and that such events should be studied carefully in other protein families. proteini mini-TGA rastlinski imunski odziv transkripcijski faktorji TGA virus krompirja Y true false true Angleški jezik Slovenski jezik Neznano 2024-07-18 14:15:07 2024-07-18 14:15:07 2024-07-19 03:45:50 0000-00-00 00:00:00 2022 0 0 Ostali avtorji: Marko Petek, Tjaša Lukan, Karmen Pogačar, Katja Stare, Erica Teixeira Prates, Daniel A Jacobson, Jan Zrimec, Gregor Bajc, Matej Butala, Maruša Pompe Novak, Quentin Dudley, Nicola Patron, Ajda Taler-Verčič, Aleksandra Usenik, Dušan Turk, Salomé Prat, Anna Coll, Kristina Gruden; str. 1934–1952 iss. 3 Vol. 191 Mar. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-03-01 578 0032-0889 10.1093/plphys/kiac579 135092739 https://doi.org/10.1093/plphys/kiac579 1 9d512494-44ee-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26912 RAZ_Tomaz_Spela_2022.pdf RAZ_Tomaz_Spela_2022.pdf 1 7E5CE539ECE1AFFC607A26125B8999D4 bca4be0bc6d2b99a4bc16e3c7bdf1415302bd5ee20374d4611667df6f0ef14db 2c98e4a9-44f0-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26913 Nacionalni inštitut za biologijo 0 0 0