20.500.12556/DiRROS-19500 Detection of four new tomato viruses in Serbia using post hoc high-throughput sequencing analysis of samples from a large-scale field survey Tomato production worldwide is affected by numerous plant virus species. The early and accurate detection of viruses is a critical step for disease control. However, the simultaneous detection of the most known tomato viruses can be difficult because of the high number and diversity of tomato-infecting viruses. Here, we have identified four new viruses in Serbia by applying target-independent small RNA high-throughput sequencing (HTS). HTS was applied on pools of samples and separate samples, in total comprising 30 tomato samples that exhibited (severe) virus-like symptoms and were collected in Serbia during three annual surveys (2011 to 2013). These samples had previously tested negative for the presence of 16 tomato viruses using targeted detection methods. Three divergent complete genome sequences of Physostegia chlorotic mottled virus were obtained from different localities, indicating for the first time that this virus is widespread in Serbia and might represent an emergent viral pathogen of tomato. The tomato torrado virus was detected at one locality with devastating yield losses. The southern tomato virus was detected at two localities, and the spinach latent virus was detected at one locality. In addition, we detected the presence of one already-known virus in Serbia, the tomato spotted wilt orthotospovirus. All the HTS results were subsequently confirmed by targeted detection methods. In this study, the successful application of post hoc HTS testing of a limited number of pooled samples resulted in the discovery of new viruses. Thus, our results encourage the use of HTS in research and diagnostic laboratories, including laboratories that have limited resources to resolve disease etiology. viruses tomato detection identification virusi patologija rastlin paradižnik odkrivanje identifikacija true false true Angleški jezik Slovenski jezik Neznano 2024-07-18 03:53:44 2024-07-19 10:03:16 2024-07-20 04:03:06 0000-00-00 00:00:00 2021 0 0 str. 2325-2332 no. 9 Vol. 105 Sept. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-10-06 581.2 0191-2917 10.1094/PDIS-09-20-1915-RE 61182723 https://apsjournals.apsnet.org/doi/10.1094/PDIS-09-20-1915-RE 1 d1cb7afa-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26901 RAZ_Vucurovic_Ana_2021.pdf RAZ_Vucurovic_Ana_2021.pdf 1 05797B731A6DA65249647008F77C16F1 0b320c879b1f2be39273e1f9928cb0be68bb43939d49d602bb51550bed6477a6 eee120d4-4594-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26967 Nacionalni inštitut za biologijo 0 0 0