20.500.12556/DiRROS-19497 TRIM28 selective nanobody reduces glioblastoma stem cell invasion Glioblastoma (GB), is the most common and aggressive malignant primary brain tumour in adults. Intra- and inter-tumour heterogeneity, infiltrative GB cell invasion and presence of therapy-resistant GB stem cells (GSCs) represent major obstacles to favourable prognosis and poor therapy response. Identifying the biomarkers of the most aggressive tumour cells and their more efficient targeting strategies are; therefore, crucial. Recently, transcription factor TRIM28 has been identified as a GB biomarker and, in this study, we have shown high expression of TRIM28 in GB and in low grade gliomas as well as higher expression in GSCs vs. differentiated GB cells, although in both cases not significant. We demonstrated significant in vitro inhibition of GB cells and GSCs invasiveness and spread in zebrafish brains in vivo by anti-TRIM28 selective nanobody NB237. TRIM28 was also enriched in GB (tumour) core and associated with the expression of stem cell genes, but was not prognostic for overall survival. However, based on the above results, we conclude that TRIM28 nanobody NB237 offers a new opportunity as a GB therapeutic tool. glioblastom TRIM28 nanoprotitelesa true false true Angleški jezik Slovenski jezik Neznano 2024-07-18 03:53:26 2024-07-19 11:17:39 2024-07-20 04:03:06 0000-00-00 00:00:00 2021 0 0 Nasl. z nasl. zaslona; Opis vira z dne 26. 8. 2021; Metka Novak, Barbara Breznik, Bernarda Majc, Barbara Hrastar, Neja Šamec, Alja Zottel, Ivana Jovčevska, Miloš Vittori, Ana Rotter, Radovan Komel, Tamara Lah Turnšek; Št. članka 26175141; str. 1-16 iss. 17 Vol. 26 Aug. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-08-25 620.3:620.1/.2 1420-3049 10.3390/molecules26175141 74227715 https://www.mdpi.com/1420-3049/26/17/5141 1 c6e2d8d6-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26898 RAZ_Porcnik_Andrej_2021.pdf RAZ_Porcnik_Andrej_2021.pdf 1 8E2816BA56D977D7F1A4215CE159DCD6 fddf23b2a77d0b6713b096b90c797a0e57505a76b2203855cd3e8cca001006c3 240a651d-459f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26986 Nacionalni inštitut za biologijo 0 0 0