20.500.12556/DiRROS-19493 Succinylation of polyallylamine: influence on biological efficacy and the formation of electrospun fibers Succinylation of proteins is a commonly encountered reaction in biology and introduces negatively charged carboxylates on previously basic primary amine groups of amino acid residues. In analogy, this work investigates the succinylation of primary amines of the synthetic polyelectrolyte polyallylamine (PAA). It investigates the influence of the degree of succinylation on the cytotoxicity and antibacterial activity of the resulting polymers. Succinylation was performed in water with varying amounts of succinic anhydride and at different pH values. The PAA derivatives were analyzed in detail with respect to molecular structure using nuclear magnetic resonance and infrared absorbance spectroscopy. Polyelectrolyte and potentiometric charge titrations were used to elucidate charge ratios between primary amines and carboxylates in the polymers. The obtained materials were then evaluated with respect to their minimum inhibitory concentration against Staphylococcus aureus and Pseudomonas aeruginosa. The biocompatibility was assessed using mouse L929 fibroblasts. The degree of succinylation decreased cytotoxicity but more significantly reduced antibacterial efficacy, demonstrating the sensitivity of the fibroblast cells against this type of ampholytic polyelectrolytes. The obtained polymers were finally electrospun into microfiber webs in combination with neutral water-soluble polyvinyl alcohol. The resulting non-woven could have the potential to be used as wound dressing materials or coatings. polyallylamine hydrochloride succinylation aqueous chemistry cytotoxicity antimicrobial effect electrospinning nanofibers mouse L929 fibroblasts Staphylococcus aureus Pseudomonas aeruginosa polialilamin hidroklorid sukcinilacija vodna kemija citotoksičnost antimikrobni učinek elektropredenje nanovlakna mišji fibroblasti L929 stafilokoki true false true Angleški jezik Slovenski jezik Neznano 2024-07-18 03:53:04 2024-07-19 11:11:43 2024-07-20 04:03:04 0000-00-00 00:00:00 2021 0 0 Nasl. z nasl. zaslona; Opis vira z dne 25. 8. 2021; Soavtorji: Matej Bračič, Silvo Hribernik, Damjan Makuc, Janez Plavec, Filip Jerenec, Sonja Žabkar, Nenad Gubeljak, Alja Štern, Rupert Kargl; Članek št.: 2840; str. 1-13 iss. 17 Vol. 13 Avg. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-08-24 677.017:543.2 2073-4360 10.3390/polym13172840 73979907 https://www.mdpi.com/2073-4360/13/17/2840 1 b8afbc9f-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26894 RAZ_Jurko_Lucija_2021.pdf RAZ_Jurko_Lucija_2021.pdf 1 5DF2289EA22087C4F15E5583B232D927 4b863a3ce24cc14b7b72d3aa0bb4edbbacdfc3f60c2f2e78209e503e5528ca60 484e6acb-459e-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26983 Nacionalni inštitut za biologijo 0 0 0